Substrate for nucleic acid amplification, and method for manufacturing same

Abstract

The present invention relates to a substrate for nucleic acid amplification, and a method for manufacturing the same. The substrate for rapid and accurate PCR analysis comprises a transparent substrate; a metal layer formed on the transparent substrate; an N-heterocyclic carbene compound having one end annealed to the surface of the metal layer; and a primer immobilized on the other end of the N-heterocyclic carbene compound.

Description

technical field [0001] The present invention relates to a substrate for nucleic acid amplification and a manufacturing method thereof. Background technique [0002] The polymerase chain reaction (PCR) method is a technique that allows large-scale amplification of specific regions of DNA or RNA in vitro. A PCR machine using this technique is used to amplify small amounts of DNA contained in blood in hospitals, research centers, etc., and is also used to diagnose various diseases including malaria, tuberculosis, hepatitis, and the like. [0003] PCR is carried out through a cycle including three steps of denaturation, annealing and extension, and the temperature is rapidly raised and lowered to be consistent with the temperature suitable for each step, which is very important for reducing the PCR reaction time. That is, PCR requires an instantaneous change in temperature to coincide with the temperature set for each step, but if a time delay occurs in the process of changing ...

AI Technical Summary

Problems solved by technology

[0004] However, conventionally used PCR devices use electric energy as a thermal energy source, and it takes a long time of more than one hour to complete the nucleic acid amplification reaction, and requires accessories including a heater and a heat sink, so there is an inconvenience that the device takes up a lot of space.

Therefore, it is a bit inconvenient to apply on-site where immediate diagnosis of diseases is required

In addition, Korean Patent Publication No. 2017-0106995 discloses a PCR system based on light energy, but there are problems in that PCR is performed in a liquid phase, it is impossible to perform multiple diagnoses in one chamber, and it is impossible to recover used primers

In particular, conventional PCR machines are not portable due to high price, equipment expansion, and long detection time (1 hour or more), and limit the point-of-care diagnosis on site

Images