Preparation method and wine brewing method of vinasse distiller's yeast
A technology of distiller's yeast and waste, which is applied in the preparation of distiller's yeast and its brewing field, which can solve the problems of increasing the cost of raw material input, and achieve the effects of cost savings, high-efficiency utilization, and simple preparation methods
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[0029]Example 1
[0030]A preparation method of distilled distiller's grains, including the following steps:
[0031]After selecting Rhizopus oryzae to inoculate the PDA medium for 48-72h, it was observed that obvious bluish black spores appeared on the surface of the hyphae. The spores were eluted with sterile water to prepare a spore liquid, which was inoculated into sterilized bran, and cultured at 30°C. After a large number of hyphae appear, they are dried / dried and crushed to obtain bran koji. Take a small part of the crushed standard sample and measure its glucoamylase activity at 1091.2U / g by Fehling's reagent method.
[0032]Five grains of sorghum, corn, rice, glutinous rice and wheat are used as raw materials, and the following quality ratios are used: sorghum 36%, corn 8%, rice 22%, glutinous rice 18%, and wheat 16% are mixed as raw materials. The raw materials are crushed, sieved through a 100-mesh sieve, and gelatinized, and the gelatinized material-water ratio is controlled to 1...
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[0038]Example 2
[0039]A preparation method of distilled distiller's grains, including the following steps:
[0040]After selecting Aspergillus niger AS3.4309 to inoculate the PDA medium for 48-72h, it was observed that obvious black spores appeared on the surface of mycelium. The spores were eluted with sterile water to prepare a spore liquid, which was inoculated into sterilized bran, and cultured at 30°C. After a large number of hyphae appear, they are dried / dried and crushed to obtain bran koji. Take a small part of the crushed standard sample and use Fehling's reagent method to measure its glucoamylase activity of 1285.7 U / g.
[0041]Five grains of sorghum, corn, rice, glutinous rice and wheat are used as raw materials, and the following quality ratios are used: sorghum 36%, corn 8%, rice 22%, glutinous rice 18%, and wheat 16% are mixed as raw materials. The raw materials are crushed, sieved through a 100-mesh sieve, and gelatinized, and the gelatinized material-water ratio is controll...
Example Embodiment
[0047]Example 3
[0048]A preparation method of distilled distiller's grains, including the following steps:
[0049]After selecting Aspergillus niger AS3.4309 to inoculate the PDA medium for 48-72h, it was observed that obvious black spores appeared on the surface of mycelium. The spores were eluted with sterile water to prepare a spore liquid, which was inoculated into sterilized bran, and cultured at 30°C. After a large number of hyphae appear, they are dried / dried and crushed to obtain bran koji. Take a small part of the crushed standard sample and use Fehling's reagent method to measure its glucoamylase activity of 1285.7 U / g.
[0050]Sorghum is used as raw material, crushed, sieved through a 100-mesh sieve, and gelatinized, and the gelatinized material-water ratio is controlled to 1:9 to prepare a yeast expansion culture medium. Weigh 1% of the commercially available Xiaoqu into it, stir it evenly and seal it and incubate it at 30°C for 48h to obtain a culture broth. According to stati...
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