Application of rana nigromaculata skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation drugs
A skin repair and anti-oxidation technology, applied in skin care preparations, skin diseases, drug combinations, etc., to achieve strong anti-oxidation activity, strong skin anti-photoaging effect, and good application prospects
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Embodiment 1
[0022] Example 1: Antioxidation and free radical scavenging effects of the bonobo frog skin repair peptide cathelicidin-NV
[0023] In this example, the ABTS+ scavenging method was used to detect the anti-oxidation and free radical scavenging effects of the bonobo frog skin repair peptide.
[0024] ABTS + ·It is a fairly stable blue-green free radical cation, which turns into colorless ABTS in the presence of antioxidants with hydrogen-donating ability. Antioxidant Peptides Clear ABTS + · The ability is measured by the change in absorbance at 734 nm. Antioxidant capacity of samples and ABTS + · The residual amount is inversely proportional to. ABTS + Residual amount (%) is calculated according to the following formula:
[0025] %ABTS + R=[(ABTS + )T / (ABTS + )t=0]×100
[0026] ABTS + ·T is for ABTS + Absorbance at 734nm after incubation with the sample in the dark for 10 minutes; ABTS + t=0 is ABTS + • Absorption value at 734 nm at initial stage. The experimental...
Embodiment 2
[0030] Example 2: The role of the bonobo frog skin repair peptide cathelicidin-NV in scavenging intracellular reactive oxygen species (ROS)
[0031] In this example, flow cytometry was used to detect the ROS content in HaCat cells mediated by ultraviolet rays (UVB), and to detect the antioxidant effect of the bonobo skin repair peptide in vivo.
[0032] UVB radiation HaCat will cause oxidative stress in cells, and at the same time cause a sharp increase in reactive oxygen species ROS in cells, resulting in cell metabolism disorders. We detected the ROS content in HaCat cells mediated by UVB by flow cytometry, and the results were as follows: figure 2 Shown: It was found that the UVB radiation treatment group significantly up-regulated intracellular ROS compared with the normal group (non-UVB radiation treatment group), and the frog skin repair peptide cathelicidin-NV could significantly reduce the intracellular ROS content. The above results fully demonstrate that cathelicidin...
Embodiment 3
[0032] UVB radiation HaCat will cause oxidative stress in cells, and at the same time cause a sharp increase in reactive oxygen species ROS in cells, resulting in cell metabolism disorders. We detected the ROS content in HaCat cells mediated by UVB by flow cytometry, and the results were as follows: figure 2 Shown: It was found that the UVB radiation treatment group significantly up-regulated intracellular ROS compared with the normal group (non-UVB radiation treatment group), and the frog skin repair peptide cathelicidin-NV could significantly reduce the intracellular ROS content. The above results fully demonstrate that cathelicidin-NV can effectively scavenge UVB-mediated ROS in HaCat cells, which further proves that it has a strong intracellular antioxidant effect. Example 3: Application of the Japanese frog skin repair peptide cathelicidin-NV in the preparation of skin anti-photoaging treatment medicine
[0033] The hairless mice were randomly divided into UVB-group: no...
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