Lactobacillus-based weight-reducing powder and preparation method thereof
A Lactobacillus and Lactobacillus fermentum technology, applied in the field of Lactobacillus-based slimming powder and its preparation, can solve problems such as limited efficacy, reduce fat accumulation, promote lipolysis and adipocyte apoptosis, and reduce serum leptin levels Effect
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[0089] The preparation method of described plantarum lactobacillus freeze-dried powder comprises the following steps:
[0090] 1) Preparation of medium: the medium is an improved MRS medium, and its formula is glucose 20-30g, beef extract 10-13g, tryptone 5-7g, soybean peptone 5-7g, yeast powder 5-6g, acetic acid Sodium 3-5g, diammonium hydrogen citrate 1-2g, dipotassium hydrogen phosphate 2-3g, magnesium sulfate 0.4-0.6g, cysteine hydrochloride 0.4-0.7g, Tween-80 1-2mL, Manganese sulfate hydrate 0.2-0.25g, water 1000mL; adjust its pH to 6.5±0.2;
[0091] 2) Preparation of bacterial strain protective agent: the formula of the bacterial strain protective agent is skim milk 60-100g / L, trehalose 80-120g / L, glycerin 15-25g / L;
[0092] 3) Inoculate Lactobacillus plantarum and / or its mutants in the fermentation substrate with 5%-10% inoculation amount to carry out fermentation culture, the fermentation temperature is 34-38°C, the fermentation time is 13-18h, and the pH of the fer...
Embodiment 1
[0105] Example 1: Lactobacillus-based slimming powder
[0106] A weight-loss powder based on Lactobacillus, including 5 parts of Lactobacillus plantarum freeze-dried powder, 5 parts of Lactobacillus fermentum freeze-dried powder, 20 parts of lotus leaf extract, 10 parts of tea polyphenols, 20 parts of konjac powder, and 10 parts of blueberry fruit powder , 13 parts of erythritol, 15 parts of inulin, 1 part of forsythia extract, 1 part of chitosan. The number of live bacteria in the freeze-dried powder of Lactobacillus plantarum and the freeze-dried powder of Lactobacillus fermentum is 1×10 11 CFU / g.
[0107] The preparation method of described plantarum lactobacillus freeze-dried powder comprises the following steps:
[0108] 1) Preparation of medium: the medium is an improved MRS medium, and its formula is glucose 20g, beef extract 10g, tryptone 5g, soybean peptone 5g, yeast powder 5.5g, sodium acetate 4g, diammonium hydrogen citrate 1.5 g, dipotassium hydrogen phosphate 2...
Embodiment 2
[0126] Example 2: Lactobacillus-based slimming powder
[0127] A weight-loss powder based on Lactobacillus, including 1 part of Lactobacillus plantarum freeze-dried powder, 1 part of Lactobacillus fermentum freeze-dried powder, 22 parts of lotus leaf extract, 12 parts of tea polyphenols, 20 parts of konjac powder, and 12 parts of blueberry fruit powder , 12 parts of erythritol, 12 parts of inulin, 1 part of forsythia extract, 2 parts of chitosan. The number of live bacteria in the freeze-dried powder of Lactobacillus plantarum and the freeze-dried powder of Lactobacillus fermentum is 5×10 11 CFU / g.
[0128] The preparation method of described plantarum lactobacillus freeze-dried powder comprises the following steps:
[0129] 1) Preparation of medium: the medium is an improved MRS medium, and its formula is 25g of glucose, 13g of beef extract, 7g of tryptone, 7g of soybean peptone, 6g of yeast powder, 5g of sodium acetate, 2g of diammonium hydrogen citrate, Dipotassium hydro...
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