A Method for Monitoring the Denaturation of Bovine Serum Albumin Based on Constant Current Differential Capacitance Signal Based on Double Carbon-Based Electrode

Abstract

The invention belongs to the technical field of protein denaturation detection, and discloses a method for monitoring the denaturation of bovine serum albumin based on constant current differential capacitance signals of double carbon-based electrodes. In the present invention, a carbon-based material silk-screened on polyethylene terephthalate is used as a working electrode, and another same electrode is used as a reference electrode and a counter electrode. The solution and the sample of the BSA solution to be monitored were respectively injected between the two electrodes, and the constant current charge and discharge analysis was carried out. The voltage was differentiated and divided by the current density to obtain a differential capacitance signal. Based on this, it was possible to identify whether the sample of the BSA solution to be monitored was denatured BSA. The method disclosed in the invention does not require external electrolytes, uses carbon-based materials from a wide range of sources as electrodes, does not cause secondary denaturation of proteins, has the advantages of low cost, easy operation, and high sensitivity, and proposes a new method for protein denaturation detection .

Description

[0001] The invention belongs to the technical field of protein denaturation detection, in particular to a method for monitoring the denaturation of bovine serum albumin (BSA) based on a constant current differential capacitance signal based on a double carbon-based electrode. Background technique [0002] Protein misfolding and aggregation seriously interfere with people's normal life, and many diseases are also caused by protein denaturation or aggregation, which brings immeasurable damage to life and production. Undoubtedly, sensitive detection of protein denaturation has important diagnostic and therapeutic implications. Bovine serum albumin is a globular protein in bovine serum albumin. It is similar in structure to human serum albumin (HSA) and has homology. Therefore, bovine serum albumin is often used as a non-redox model protein to study proteins. Structural changes. [0003] The current methods for protein secondary structure and denatured protein secondary structure...

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Problems solved by technology

[0003] The methods currently used for the analysis of protein secondary structure and denatured protein secondary structure include X-ray diffraction, circular dichroism, fluorescence spectroscopy, ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy, etc., but these methods are generally tested Higher cost, more complex data analysis, more troublesome sample processing

The electrochemical detection method has always had the advantages of convenient use, high measurement sensitivity, simple maintenance and low cost. Mercury-containing electrode, and the potential range of the analysis is negative, so it is easy to cause secondary denaturation of proteins

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