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Biocontrol agent for preventing and treating garlic diseases and its preparation method and application

A biocontrol agent and bacterial fermentation technology, which is applied in the field of agricultural microorganisms, can solve the problems of incomplete degradation, narrow antibacterial spectrum, pesticide residues, etc., and achieve the effects of eco-friendliness, plant growth promotion, and easy production and preparation

Active Publication Date: 2022-04-22
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antibacterial spectrum of a single chemical agent is narrow, and it is difficult to cure diseases caused by various pathogenic bacteria
On the one hand, the long-term use of chemical agents makes some pathogenic bacteria develop drug resistance, on the other hand, it affects the growth of some beneficial microorganisms in the soil
Chemical agents also have problems such as refractory or incomplete degradation, which pose potential ecological risks to the environment and cause pesticide residues

Method used

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  • Biocontrol agent for preventing and treating garlic diseases and its preparation method and application
  • Biocontrol agent for preventing and treating garlic diseases and its preparation method and application
  • Biocontrol agent for preventing and treating garlic diseases and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The medium used to prepare the seed liquid is liquid LB medium: peptone 10g, yeast powder 5g, NaCl 10g, supplemented with deionized water to 1L, pH 7.0.

[0031] Fermentation medium for Pseudomonas aeruginosa: glucose 35g, NaNO 3 3.0g, KH 2 PO 4 3.0g, MgSO 4 ∙7H 2 O1.0 g, CaCl 2 ∙2H 2 O 0.15g, K 2 HPO 4 ∙3H 2 O 4.0g, KCl 0.5g, add deionized water to 1L.

[0032] Fermentation medium of Bacillus subtilis: 30 g of sucrose, NaNO 3 3.0g, KH 2 PO 4 3.0g, MgSO 4 ∙7H 2 O1.0g, CaCl 2 ∙2H 2 O 0.15g, K 2 HPO 4 ∙3H 2 O 4.0g, KCl 0.5g, NaCl 0.5g, add deionized water to 1L.

[0033] The above reagents were weighed with an electronic balance to prepare the corresponding culture medium; after packaging, placed in 115 ℃ for 30min sterilization.

[0034] The strains used in this example are Pseudomonas aeruginosa CGMCC NO. 21276 and Bacillus subtilis CGMCC NO. 13461 as examples.

[0035] After the medium was cooled to 20°C to 40°C, use an inoculation loop to scra...

Embodiment 2

[0038] Take 2 kinds of single bacteria fermentation broths in Example 1, 20 mL of single bacteria fermentation broth of Pseudomonas aeruginosa, and 40 mL of single bacteria fermentation broth of Bacillus subtilis, and mix them evenly in a 3L beaker; add 2940mL of deionized water , diluted 50 times to obtain the ready-to-use biocontrol agent.

[0039] Take 200ml of the above-obtained biocontrol agent, add it to 1kg of soil while turning the soil, mix it with mud, take 100g of the mixed soil, take a garlic clove, wrap the garlic clove with soil, and make the outer side is the soil and the center is the garlic clove. Mud balls for sowing garlic.

Embodiment 3

[0041] The biocontrol agent obtained in Example 2 was used to act on garlic pathogenic bacteria and pathogenic fungi, and the size of the inhibition zone was determined to evaluate the bacteriostatic effect of the biocontrol agent of the present invention on different garlic pathogenic bacteria.

[0042] Prepare LB liquid medium and potato dextrose liquid medium, add 2% agar powder for sterilization, prepare LB agar medium plate and PDA medium (potato dextrose agar medium) plate, respectively for culturing garlic pathogenic bacteria and garlic Pathogenic fungi. The garlic pathogenic bacteria to be tested are the phytopathogenic bacteria (strains L2#, L5#, L6#, L7#) and phytopathogenic fungi (strains P4#, P12#, P14#, P16#) screened by the laboratory from the diseased garlic .

[0043] Use LB liquid medium and potato dextrose liquid medium to prepare the seed liquid of the bacteria to be tested and the fungi to be tested, respectively, and dilute the seed liquid to 10 5 After ...

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Abstract

The invention provides a biocontrol agent for preventing and treating garlic diseases, a preparation method and application thereof. The biocontrol bacteria agent is a compound bacteria agent composed of Pseudomonas aeruginosa and Bacillus subtilis, which is prepared by mixing the two kinds of single bacteria fermented liquid in proportion after being fermented by a single bacteria. Pseudomonas aeruginosa is named Pseudomonas aeruginosa , has been preserved in the China General Microorganism Culture Collection Management Center on December 1, 2020, and the preservation number is CGMCC NO.21276. After the biocontrol bacterium agent of the present invention is diluted with water, it is mixed with soil in an appropriate proportion to make a mud, and the even soil is taken to wrap garlic seeds for garlic sowing. The garlic biocontrol bacteria agent of the invention can effectively inhibit the growth of pathogenic bacteria and pathogenic fungi of garlic, and can be applied to prevent and treat garlic diseases caused by pathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a biocontrol agent for preventing and treating garlic diseases and a preparation method and application thereof. Background technique [0002] Garlic is one of the common condiments and also has high nutritional and medicinal value. Garlic is an important economic crop. Garlic has a long history of cultivation in my country. The domestic and foreign markets of garlic have great potential and great demand. Due to continuous planting in successive years, unscientific management of fertilizer and water, and other factors, garlic diseases frequently occur, which seriously affects the yield and quality of garlic, and endangers the garlic planting industry and garlic deep processing industry. [0003] Garlic diseases garlic blight, garlic purpura, garlic leaf blight, garlic soft rot and other diseases. The diseases in the whole growth period of garlic include soft...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01N63/22A01P1/00A01P3/00A01C1/06C12R1/125C12R1/385
CPCC12N1/20A01N63/27A01N63/22A01C1/06
Inventor 赵峰王秉鑫袁梦林高配科孔勇
Owner QUFU NORMAL UNIV
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