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Preparation method of pigskin collagen with certain molecular weight range

A technology of collagen and molecular weight, which is applied in the preparation method of peptides, chemical instruments and methods, animal/human protein, etc., can solve the problems of excessive molecular weight and difficult absorption of pigskin collagen, and achieve the improvement of oil content and improvement Good skin elasticity and water solubility

Inactive Publication Date: 2021-04-06
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing pigskin collagen with a certain range of molecular weight, so as to solve the problem in the above-mentioned background technology that the molecular weight of pigskin collagen is too large to be absorbed by the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing pigskin collagen with a certain range of molecular weight, the specific steps are as follows:

[0038] S1: pigskin pretreatment;

[0039] S2: enzymatic membrane separation;

[0040] S3: decolorization and deodorization treatment;

[0041] S4: Freeze drying.

[0042] The concrete steps of pigskin pretreatment in the present embodiment are as follows:

[0043] S11: remove the hair from fresh pigskin, wash and remove the epidermis, and remove the fat layer;

[0044] S12: Add H in the ratio of 1:20 2 o 2 NaOH solution, stirred and soaked at 20°C for 1 hour, rinsed with distilled water until neutral, and drained;

[0045] S13: adding liquid nitrogen to the processed pigskin fragments and grinding them into powder.

[0046] Specifically, in step S12, H 2 o 2 The added amount of NaOH is 0.8%, and the concentration of NaOH solution in step S12 is 0.01mol / L.

[0047] The specific steps of enzymatic membrane circulation separation in the present e...

Embodiment 2

[0061] A method for preparing pigskin collagen with a certain range of molecular weight, a method for preparing pigskin collagen with a certain range of molecular weight, the specific steps are as follows:

[0062] S1: pigskin pretreatment;

[0063] S2: enzymatic membrane separation;

[0064] S3: decolorization and deodorization treatment;

[0065] S4: Freeze drying.

[0066] The concrete steps of pigskin pretreatment in the present embodiment are as follows:

[0067] S11: remove the hair from fresh pigskin, wash and remove the epidermis, and remove the fat layer;

[0068] S12: Add H in the ratio of 1:20 2 o 2 NaOH solution, stirred and soaked at 15°C for 1 hour, rinsed with distilled water until neutral, and drained;

[0069] S13: adding liquid nitrogen to the processed pigskin fragments and grinding them into powder.

[0070] Specifically, in step S12, H 2 o 2 The addition amount of NaOH is 1%, and the concentration of NaOH solution in step S12 is 0.01mol / L.

[007...

Embodiment 3

[0085] A method for preparing pigskin collagen with a certain range of molecular weight, the specific steps are as follows:

[0086] S1: pigskin pretreatment;

[0087] S2: enzymatic membrane separation;

[0088] S3: decolorization and deodorization treatment;

[0089] S4: Freeze drying.

[0090] The concrete steps of pigskin pretreatment in the present embodiment are as follows:

[0091] S11: remove the hair from fresh pigskin, wash and remove the epidermis, and remove the fat layer;

[0092] S12: Add H in the ratio of 1:20 2 o 2 NaOH solution, stirred and soaked at 20°C for 1 hour, rinsed with distilled water until neutral, and drained;

[0093] S13: adding liquid nitrogen to the processed pigskin fragments and grinding them into powder.

[0094] Specifically, in step S12, H 2 o 2 The added amount of NaOH is 1.2%, and the concentration of NaOH solution in step S12 is 0.02mol / L.

[0095] The specific steps of enzymatic membrane circulation separation in the present e...

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PUM

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Abstract

The invention relates to the technical field of colla corii asini production, in particular to a preparation method of the pigskin collagen with certain molecular weight range. The method comprises the following specific steps: pigskin pretreatment, enzymolysis membrane cyclic separation, decoloration and deodorization treatment and freeze drying. Pigskin pretreatment, enzymolysis membrane cyclic separation, decoloration and deodorization treatment and freeze drying are performed through specific combined process steps and parameter conditions to obtain collagen powder with the molecular weight of 2-5KD. The collagen powder has the characteristics that a triple helix structure for preparing pigskin collagen is not damaged; the original biological activity is still maintained; the main component is 2-5KD collagen powder; the pigskin collagen is derived from vertebrate collagen and human body, and the skin has good homology and compatibility; the collagen powder can be quickly dissolved into the epidermis after being applied to the skin and can be quickly dissolved into the epidermis after being applied to the skin, and the collagen polypeptide with the molecular weight lower than 5kDa has various physiological activities such as blood pressure reduction and oxidation resistance.

Description

technical field [0001] The invention relates to the technical field of donkey-hide gelatin production, in particular to a method for preparing pigskin collagen with a certain molecular weight. Background technique [0002] Collagen is a biological polymer, the main component of animal connective tissue, and the most abundant and widely distributed functional protein in mammals, accounting for 25% to 30% of the total protein, and some organisms even reach more than 80% . Collagen has been widely used in food, medicine, tissue engineering, cosmetics and other fields due to its good biocompatibility, biodegradability and biological activity. Pig skin, a by-product of pork production, is heat-treated during pork processing in meat factories and cannot be used for leather manufacturing. Wherein the protein content is as high as more than 33%, and the collagen protein content is about 88%, and its nutritional value is very considerable. Due to the special and complex spatial st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/14C07K1/34
CPCC07K14/78C12P21/06
Inventor 牛海涛梁烨王永华李丹
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV