Preparation method of chitosan oligosaccharide and weight-reducing tablet
A technology of chitosan and chitosan, which is applied in the field of pharmaceutical preparations, can solve problems such as chitosan that is not suitable for weight loss, and achieve the effects of increasing lipid excretion, reducing adipose tissue hyperplasia, and ensuring safety
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[0025] The invention provides a preparation method of chitosan oligosaccharide, comprising: S1) swelling chitosan in an acidic aqueous solution, then adding chitosanase, heating for enzymolysis to obtain an enzymolysis solution; S2) dissolving the enzyme After the solution is treated with enzyme inactivation and clarification, the liquid chitosan oligosaccharide with number average molecular weight ≤ 1Kda is obtained.
[0026] Oligochitosan with a number average molecular weight of ≤1KDa is a low polymer composed of 3 to 6 glucosamine and N-acetylglucosamine connected by β-1,4 glycosidic bonds. It has good water solubility and the absorption rate in the human intestine is close to that of 100%, non-toxic and side effects on the human body, with unique physiological activities and functions.
[0027] The present invention has no special limitation on the sources of all raw materials, which can be commercially available. Wherein, preferably the degree of deacetylation of the ch...
Embodiment 1
[0044] A kind of chitosan oligosaccharide of number average molecular weight≤1KDa and the preparation method of slimming tablet, its preparation steps are as follows:
[0045] (1) chitosan swelling:
[0046] Get the chitosan 5Kg that deacetylation degree ≥ 90% is in 150L reactor, add 95L purified water and be mixed with the chitosan solution of 6% (w / w), be 4.5 with the pH of lactic acid regulation solution, constantly stir, in Swell in a water bath at 50°C for 5h.
[0047] (2) Preparation of liquid chitooligosaccharides with number average molecular weight ≤ 1KDa:
[0048] Add chitosanase 1.65×10 5 1, stirred at 50°C for 3h, and the obtained enzymolysis solution was kept at 78°C in a water bath for 10min to inactivate the enzyme. After the solution was cooled, 0.4Kg of diatomaceous earth was added, stirred evenly, and filtered to obtain a clear liquid chitooligosaccharide with a number average molecular weight of 930Da.
[0049] (3) Preparation of solid chitosan oligosacch...
Embodiment 2
[0054] A kind of chitosan oligosaccharide of number average molecular weight≤1KDa and the preparation method of slimming tablet, its preparation steps are as follows:
[0055] (1) chitosan swelling:
[0056] Get deacetylation degree ≥ 90% chitosan 50Kg in 5T reactor, add 2450L purified water and be mixed with the chitosan solution of 2% (w / w), be 5.5 with the pH of lactic acid regulation solution, constantly stir, in Swell in a water bath at 57°C for 3h.
[0057] (2) Preparation of liquid chitooligosaccharides with number average molecular weight ≤ 1KDa:
[0058] Add chitosanase 1.5×10 6 1, stirred at 57° C. for 5 h, and the obtained enzymolysis solution was kept at 85° C. in a water bath for 12 minutes to inactivate the enzyme. After the solution was cooled, 20 Kg of diatomaceous earth was added, stirred evenly, and filtered to obtain a clear liquid chitosan oligosaccharide with a number average molecular weight of 890 Da.
[0059] (3) Preparation of solid chitosan oligosa...
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