The culture medium for producing elamycin e by utilizing Streptomyces deep-sea and its preparation method
A technology of deep-sea streptomyces and eleomycin, which is applied in the field of microbial fermentation medium and culture medium of deep-sea streptomyces, can solve the problems of low yield of eleomycin E, and is conducive to popularization and application and increased production , cheap effect
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Embodiment 1
[0040] 1) Configuration of fermentation medium
[0041]Accurately weigh 120 g of glucose, 20 g of soybean flour, 2.4 g of corn steep liquor, 6 g of sodium chloride, 9.6 g of sodium nitrate, 6.4 g of ammonium sulfate, and 0.24 g of potassium dihydrogen phosphate, dissolve them in deionized water, and dilute to 1000 mL. After adjusting the pH to 7.2-7.4 with 3M sodium hydroxide solution, 9.6 g of calcium carbonate was added. Next, it was sterilized at 121° C. for 20 minutes after being dispensed into shake flasks.
[0042] 2) Fermentation operation process
[0043] After thawing the spore liquid of Streptomyces abyssus SCSIO ZH16△ilaR cryopreserved at -20°C, it was streaked in solid medium and grown for 7-14 days to obtain a fresh solid medium rich in spores. The size of about 0.5cm is excavated from the solid medium of fresh Streptomyces deep-sea SCSIOZH16△ilaR 2 The agar block was inoculated into a 250 mL shake flask containing 25 mL of seed medium, and was fermented and cu...
Embodiment 2
[0048] 1) Configuration of fermentation medium
[0049] Accurately weigh 120 g of glucose, 20 g of yeast extract, 2.4 g of corn steep liquor, 6 g of sodium chloride, 9.6 g of sodium nitrate, 6.4 g of ammonium sulfate, and 0.24 g of potassium dihydrogen phosphate, dissolve them in deionized water, and set the volume to 1000 mL. After adjusting the pH to 7.2-7.4 with 3M sodium hydroxide solution, 9.6 g of calcium carbonate was added. Next, it was sterilized at 121° C. for 20 minutes after being dispensed into shake flasks.
[0050] 2) Fermentation operation process
[0051] The fermentation operation process is the same as that of Example 1.
[0052] 3) Yilaimycin E yield detection
[0053] Same as the yield detection of elamycin E in Example 1.
[0054] Finally, the yield of the target product elamycin E was 121.55 mg / L, and the biomass was 27.6 g / L.
Embodiment 3
[0056] 1) Configuration of fermentation medium:
[0057] Accurately weigh soluble starch 120g / L, soybean flour 20g / L, corn steep liquor 2.4g / L, sodium chloride 6g / L, sodium nitrate 9.6g / L, ammonium sulfate 6.4g / L, potassium dihydrogen phosphate 0.24g / L, dissolved in deionized water, and the volume was adjusted to 1000 mL. After adjusting the pH to 7.2-7.4 with 3M sodium hydroxide solution, 9.6 g of calcium carbonate was added. Next, it was sterilized at 121° C. for 20 minutes after being dispensed into shake flasks.
[0058] 2) Fermentation operation process
[0059] The fermentation operation process is the same as that of Example 1.
[0060] 3) Yilaimycin E yield detection
[0061] Same as the yield detection of elamycin E in Example 1.
[0062] Finally, the yield of the target product Elenomycin E was 54.05mg / L, and the biomass was 23.5g / L.
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