Enterococcus faecium, drug for preventing or treating tumor and application
A technology of enterococcus lactic acid and medicine, which is applied in the field of medicine and biology, can solve the problems of failure, side effect treatment, etc., achieve the effect of increasing secretion and realizing the effect of tumor prevention or treatment
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[0050]Example 1
[0051]This example provides a lactic acid intestinal bacteria, separated, purified, and colony identification as follows.
[0052]Isolation, purification of strains MNC-168.
[0053](1) Dividen saline in a biosafety cabinet in a 10 ml centrifuge tube; transfer anaerobic blood plate and sterile saline in an anaerobic table (H35 Hypoxystation, Whitley 24 hours in advance, and will be 5 ~ 7 sterile glass beads poured into the resected anaerobic blood plate. Anaerobic Blood Plate is purchased from Jiangmen Kailin Trading Co., Ltd., BIO-CARING.
[0054](2) After Munne (Guangzhou) Biotechnology Co., Ltd. (donor number 403051) signed the informed consent of the company to collect the feces, take the exuberant sample 1G in anaerobic operating table to sway 1min, mix , Draw 1 ml sample to 9 ml of physiological saline, mix well to 10-1Diluent, dilute to 10 gradients-6Diluent for use in a plate.
[0055](3) Take 10 μL of 10 in step (2)-6The dilution fluid is in an anaerobic blood plate, and...
Example Embodiment
[0070]Example 2
[0071]The present embodiments are prepared, sequencing, assembly, and analysis of the MNC-168 original strain obtained by the first embodiment.
[0072]The genome of the MNC-168 original strain is sequence fragmentation by ultrasonic method, fragmentation length range ~ 350 bp, and then utilizes a standard DNA construction library kit (NEBUltraTM Construct the Illumina sequencing library. The build-up sequencing library will be used to sequencing the dual-end 150bp using Novaseq (Illumina). The sequencing is 2.85 Gbp data, with the ratio of Q20: 96.605%.
[0073]Genomic raw sequencing data is filtered with FASTP (Version: 0.20.0), filter parameters: "- Q 15-L 50". The filtered raw data uses Spades (Version: V3.13.1) for genome assembly, assembling parameters "-isolate - Cov-Cutoff 10". The genome assembly was equipped with a total length of 2.84 mbp, and N50 length was 79.90 kbp, and the GC content was 38.17%. The genomic similarity is the highest strain of ENTEROCCCUS Lact...
Example Embodiment
[0082]Experimental Example 1
[0083]In this experiment, the effect of MnC-168 to macrophages obtained by separation of MNC-168.
[0084]Recent studies have shown that cancer patients have an important influence on the effects of tumor immunotherapy, while regulating and utilizing intestinal microorganisms are also known as tumor immunotherapy. It has been proven that there is a plurality of mechanisms with direct interaction between intestinal microorganisms and immune cells, while dendritic cells and macrophages in intestinal lymphoid tissue are key target cells of immunomodulatory microorganisms, especially in small intestines, Due to the large intestinal surface area, the mucus layer is thin, so that the microorganism can be in close contact with immune cells. Different specific strains in the microbial flora can inhibit or activate the immune response of the entire body.
[0085]Macrophages are the heterogeneous population of the neat cells in the natural immune system, involved in a va...
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