Primer probe set, kit and detection method for multiple detection of human parainfluenza virus subtypes based on fluorescence RMA method
A human parainfluenza virus, multiple detection technology, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of complex operation procedures, inability to detect parainfluenza virus, and many false negatives
Pending Publication Date: 2021-04-09
济南国益生物科技有限公司
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Abstract
The invention belongs to a detection method for human parainfluenza virus subtypes, and particularly relates to a primer probe group, kit and detection method for multiple detection of human parainfluenza virus subtypes based on a fluorescence RMA method. The kit comprises a detection tube containing an amplification reaction reagent, a buffer solution, magnesium acetate, a standard positive plasmid and sterile double distilled water, wherein the amplification reaction reagent comprises a primer and probe of HPIV1-3, M-MLV reverse transcriptase, escherichia coli RecA protein, UvsY protein, single-stranded binding protein GP32, Bst polymerase, exonuclease III, polyethylene oxide, trehalose, mannitol, ATP, dNTPs, creatine kinase and phosphocreatine; and the standard positive plasmid is a recombinant plasmid obtained by connecting an HN gene segment of HPIV1 with a pMD18-T vector.
Application Domain
Microbiological testing/measurementDNA/RNA fragmentation
Technology Topic
PhosphorylcreatineExonuclease III +16
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