Metarhizium lepidiotae, microbial insecticide, preparation method and application
A microbial insecticide, Metarhizium anisopliae technology, applied in the field of preparation of microbial insecticides, Metarhizium anisopliae, can solve the problems of high toxicity of chemical pesticides and pesticide residues, and achieve resistance to pesticides, high lethality, The effect of promoting plant growth
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Embodiment 1
[0050]In this embodiment, the squama is preserved, and the preservation name is the Matrodhiziumlepidiotae MN114915, the preservation number is, the preservation time is, the deposit address is, the classification name is.
[0051]The separation of the above strains, the purification method includes the steps of sequential:
[0052]Soil: After collecting the agricultural soil of Guangxi, after drying, remove the soil samples to remove impurities, weigh the dried soil 10G to load the filtration device.
[0053]Washing: Washing soil with tap water, soil particles flow by 1mm brass net and two polypropylene filters. The bottom of the soil particles were collected, put it in a sterile 50 ml centrifuge tube, add 50 ml of sterile water, and the scroll mixer is suspended. Then centrifuge at 10,000 rpm, poured down, washed with sterile water, centrifugally, and washed 3 times.
[0054]The precipitate was then diluted: a sterile carboxymethylcellulose solution solution was added to the proportion of wat...
Embodiment 2
[0058]The strains obtained by separated from Example 1 were separately analyzed and molecular biological identification.
[0059]Morphological identification:
[0060]The isolated squamous spaclasmia was inoculated at 28 ° C for 15 ° C on the SDAY medium plate at 28 ° C for 15 days. The white mycetes were collected into a production piece after 4 to 5 days, and the bacteria and the spore pendulum form were observed under the microscope; and the spores were collected from 10 to 12 days to prevent the size and morphology of the spore. Forms of colonies in the record colonies in 15 days.
[0061]figure 1 As shown in the vaccination 4 to 5 days, the microgram after the white mycelium production is picked, and the magnification is 400 times.figure 2 The microgram after the inoculation of the spore production is picked up after 10 to 12 days, and the magnification is 400 times.image 3 A front view of the colonies of the Mn113832 strain for 15 days,Figure 4 Rear view of the colony form of the Mn113...
experiment example 1
[0078]In this first experiment, the strain MN113832, MN114915 separated from Example 2 was respectively biological assays, and the effect of the temperature on the strain MN113832, MN114915, and was measured on the growth rate, spore, spore germination rate of colonies.
[0079](1) The effect of temperature on the growth of strains.
[0080]The cultured squamous spore spore was washed with 0.1% Tween 8, and 10 μl was taken into a new SDAY medium, respectively at 20 ° C, 25 ° C, 28 ° C, 30 ° C, respectively, five different temperatures at 37 ° C. Temperature culture, measure the diameter of colonies every day, and three repetitions are set each temperature treatment group.
[0081](2) Determination of colony growth rate.
[0082]The cultured squamous spore spore was washed with 0.1% Tween 80, and 10 μl was taken to the new SDAY medium, and three repetitions were determined, and the diameter was measured and data was recorded until the colonies were full of medium.
[0083](3) Determination of spore...
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