A surface-enhanced Raman scattering detection substrate, system and preparation method thereof and its application in cancer diagnosis
A surface-enhanced Raman and substrate technology, applied in the fields of biological detection and cancer diagnosis, can solve the problems of inability to achieve orderly distribution of SERS probes, complicated SERS substrate preparation process, detection stability and order damage, etc. The effect of uniformity, excellent efficiency and low error rate
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[0040] In one or more embodiments of the present invention, the present invention provides a method for preparing a surface-enhanced Raman scattering detection substrate, the steps of which include: metal hot embossing process, hydrothermal growth of silver nanoparticles, spin-coated graphite Oxygen film, scrape coating functional magnetic bead system, in some embodiments, can be as figure 2 The operation shown in the procedure obtains a homogeneous substrate that can be directly subjected to Raman testing. In some more specific implementations, the preparation method includes: using hot embossing technology on the surface of the metal substrate 110 to obtain a uniform microlens pit array 120, performing plasma treatment on the copper sheet, and using a hydrothermal method on the A layer of silver nanoparticles 130 is grown on the inner surface of the pit structure, and then a layer of graphene film 140 is coated on the surface by a spin coating process, and finally the funct...
preparation example 1
[0046] A functional magnetic bead system, the structure of which is immune functional magnetic beads-exosomes-4-MBA@nano-silver particles, obtained by the following steps: combining nano-silver particles with 4-MBA, and then activating 4-MBA with NHS and EDC The carboxyl group on which the GPC-1 antibody is linked to form a SERS tag. Then, streptavidin magnetic beads (2 μm in diameter) were combined with CD-63 antibody to form CD-63 functionalized magnetic beads and capture exosomes. Mix and incubate the SERS label with the functional magnetic beads after capturing exosomes, purify the magnetic beads with a magnetic frame, and obtain the functional magnetic bead system after washing. The top view of its scanning electron microscope photo is as follows image 3 shown.
preparation example 2
[0048] A surface-enhanced Raman scattering detection substrate, which is prepared by the following steps: using hot embossing technology on the surface of a metal substrate 110 (copper sheet) to obtain a uniform microlens pit structure array 120, and performing plasma treatment on the copper sheet, A layer of silver nanoparticles 130 is grown on the inner surface of the pit structure by a hydrothermal method, and then a layer of graphene film 140 is coated on the surface by a spin coating process, and finally the functional magnetic bead system 150 (prepared in Example 1) is scraped-coated Enter the pit structure array to obtain a uniform substrate that can be directly used for Raman testing. In the metal hot embossing process, the imprinting speed is 150r / min, the imprinting gap is 0.1mm, and the roller temperature is 100°C; in the spin coating process, the spin coating speed is 200r / min; The coating speed is 3mm / s.
[0049] Among them, in the microlens pit structure array i...
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