Human bone marrow mesenchymal stem cell induction culture medium and induction method
A technology of bone marrow mesenchyme and induction medium, which can be applied in the direction of cell culture active agent, bone/connective tissue cells, culture process, etc. Achieve the effect of applicable industrialization and clinical application, avoiding risks and uncertainties, and efficient clinical treatment research
Active Publication Date: 2021-04-30
北京益华生物科技有限公司
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However, the type of factor components after induction is relatively single, only the secretion of GDNF is increased, and it does not increase the secretion of BDNF related to the treatment of ischemic stroke. In addition, in the treatment of ischemic stroke, umbilical cord mesenchymal stem cells, The effect is not as obvious as that of bone marrow mesenchymal stem cells, and the level of factor secretion induced by the induction scheme is not high, and it cannot be secreted at a high level for a long time; in addition, low-generation (3-4 generation) is generally used cells, so that the amount of cells that can be induced per unit of primary cells is very limited
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Embodiment 1
[0034] A human-derived bone marrow mesenchymal stem cell induction medium, the induction medium is composed of α-MEM medium and additives, in terms of final concentration, the additives are composed of 20ng / ml epidermal growth factor, 200ng / ml regulatory protein β1, Composed of 1mM trehalose and 2mM dibutyryl cyclic adenosine monophosphate.
Embodiment 2
[0036] A human-derived bone marrow mesenchymal stem cell induction medium, the induction medium is composed of α-MEM medium and additives, in terms of final concentration, the additives are composed of 5ng / ml epidermal growth factor, 10ng / ml regulatory protein β1, Composed of 100nM trehalose and 100nM dibutyryl cyclic adenosine monophosphate.
Embodiment 3
[0038] A human-derived bone marrow mesenchymal stem cell induction medium, the induction medium is composed of α-MEM medium and additives, in terms of final concentration, the additives are composed of 100ng / ml epidermal growth factor, 2000ng / ml regulatory protein β1, Composed of 10000nM trehalose and 10000nM dibutyryl cyclic adenosine monophosphate.
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The invention discloses a human bone marrow mesenchymal stem cell induction culture medium and an induction method. The induction culture medium is composed of an alpha-MEM culture medium and an additive, wherein the additive is composed of 5-100 ng / ml epidermal growth factor, 10-2000 ng / ml regulatory protein beta1, 100-10000 nM trehalose and 100-10000 nM dibutyryl cyclic adenosine monophosphate according to the final concentration. According to the induction culture medium and the induction method for the human bone marrow mesenchymal stem cells, the secretion amounts of brain-derived neurotrophic factors, glial cell-derived neurotrophic factors, hepatocyte growth factors and vascular endothelial growth factors of the bone marrow mesenchymal stem cells can be obviously increased, and secreted factors are rich in variety and can be stably secreted for a long time; and risks and uncertainty caused by other methods such as gene modification are avoided through a cytokine induction method, and the method is more suitable for industrialization and clinical application.
Description
technical field [0001] The invention relates to the technical field of biomedicine, in particular to a human bone marrow mesenchymal stem cell induction medium and an induction method. Background technique [0002] With the progress of stem cell research, stem cell transplantation has brought a new dawn for the effective treatment of ischemic stroke. Among various types of stem cells, bone marrow mesenchymal stem cells have become an ideal seed cell for transplantation due to their advantages of convenient material extraction, low immunogenicity, and strong expansion ability. Multiple studies have shown that bone marrow mesenchymal stem cell transplantation can improve neurological dysfunction caused by ischemic stroke to varying degrees. Kvortsova et al. transplanted BM-MSCs into the rat model of focal cerebral ischemia through intravenous injection, and found that the transplanted bone marrow mesenchymal stem cells were mainly distributed in the ischemic stroke site, whic...
Claims
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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2500/34C12N2500/40C12N2501/11C12N2501/13
Inventor 姜粉军仇志强成彦文何子周慧
Owner 北京益华生物科技有限公司