Preparation method of material for identifying age of long-fin true shark

Abstract

The invention discloses a preparation method of a material for identifying the age of long-fin true shark, and the method selects a vertebra material and comprises the following steps: (1) extraction: taking about 3-5 sections of vertebrae from a first dorsal fin, and carrying out cryopreservation; (2) cleaning: cutting off muscles and connective tissues on the vertebrae, washing with distilled water, then putting the vertebrae into boiling water, boiling for 5-10 minutes, removing the connective tissues which are difficult to remove, if the connective tissues are still attached to the vertebrae, putting the vertebrae into a 30% H2O2 solution, performing soaking for about 1-2 hours, performing soaking with distilled water, repeatedly performing washing, and performing drying; (3) rough grinding: carrying out rough grinding on a metallographic grinder by using 120 grits waterproof wear-resistant abrasive paper, and respectively grinding the two surfaces to be close to the central position to form a vertebra slice with the thickness of about 3-5mm. According to the method, the longitudinal section of the true shark vertebra of the long fin is ground, force is exerted evenly, the ground and polished vertebra slice is sealed through the sealing agent, and it is guaranteed that the wheel lines can be kept for a long time.

Description

technical field [0001] The invention relates to a preparation method of a material for age identification of marine organisms, in particular to a preparation method of a material for age identification of whitetip sharks. Background technique [0002] The longfin shark (Carcharhinus longimanus) belongs to the family Carcharhinidae (Carcharhiniformes) of the order Carcharhiniformes. One of the tuna longline and fishing species. However, with the intensification of overfishing and the deterioration of the marine environment in recent years, the resources of whitetip sharks have declined severely, and are now listed as endangered species by the International Union for Conservation of Nature (IUCN). [0003] Fish age information is the basis for fishery resource assessment. In the study of population dynamics, accurate age and growth information can help predict fish resource changes. The vertebra has the characteristics of stably storing growth information, and is the most ef...

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for preparing materials for age identification of longfin sharks, the purpose of which is to make the ring pattern on the complete vertebra clearer through the dyeing method, and to solve the problem that small vertebra slices are not easy to grind, easily damaged during the grinding process, Problems such as low definition of wheel lines after grinding

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