A kind of method for disinfection and rapid multiplication of stem section of Millennium paulownia
A stem segment and bud-carrying technology, which is applied in the field of disinfection and rapid multiplication of Millennium paulownia stem segments, can solve the problems of difficult sterilization of Millennium paulownia explants and insufficient technology for regenerating plants, and achieve shortened cultivation period, good induction effect, and good seedling growth. vigorous growth effect
Active Publication Date: 2022-06-03
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Problems solved by technology
[0012] The primary purpose of the present invention is to provide a disinfection method for the semi-lignified bud stem section of the Millennium tung tree in the current year, aiming at the problem that it is difficult to sterilize the explants of the Millennium tung tree and the technology of obtaining regenerated plants through tissue culture is insufficient
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Embodiment 1
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[0065]
[0066] Finally confirm that the preferred rooting medium of the present invention is: 1 / 2MS+2.0mg / L IBA.
[0068] D1:201310271826.7 A kind of method of tung tree leaf regeneration plant
[0076]
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The invention provides a method for sterilizing and rapidly proliferating the stem section of the thousand-year-old tung tree. Including explant disinfection, axillary bud induction, rooting culture and other processes. The invention overcomes the problems of high pollution rate and difficult cultivation of the first-generation culture of the stem section of Millennium Tung tree, and has the advantages of low contamination rate of explants, fast propagation rate and short cultivation period, and not only provides rapid propagation of excellent aseptic seedlings of Millennium Tung tree. It opened up a way, and provided the possibility for the industrialized seedling raising of Millennium Tung tree and the transgenic technology in the future.
Description
A method for disinfection and rapid proliferation of stem sections of Millennium Tung technical field The invention belongs to the field of Tung Tung tissue culture, be specifically related to a kind of method for disinfection of Tung tung stem section and rapid proliferation. Law. Background technique Millennium tung (Vernicia montana) is Euphorbiaceae (Euphorbiaceae) oil tung genus (Vernicia Lour.) The deciduous tree is a typical south subtropical tree species, in my country, north latitude 22°15'~34°30', east longitude 99°40'~122°07' Both are cultivated and distributed, and the main cultivation areas are Fujian, Guangdong, Guangxi, Jiangxi, Hunan and other provinces. The oil content of thousand-year-old dried tung seeds is 60% ~70%, one of the cultivation purposes is to extract oil from seeds, which can be developed into biomass energy forest. It has good fast-growing cold resistance As a timber species, it also has good development potential, especially in ar...
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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/00Y02P60/40
Inventor 李泽张慧马芳芳刘洋谭晓风曾艳玲张琳王珊杨昕悦高晓惠
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY