Traditional Chinese medicine hair-blacking composition and preparation thereof
A technology of composition and traditional Chinese medicine, which is applied in the direction of drug combination, drug delivery, hair care, etc., can solve the problems of complex ratio of medicinal materials, cumbersome preparation methods, etc., and achieve good hair blackening effect, simple preparation process, and convenient use
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Embodiment 1
[0025] In vitro experiments: Including Eclipta, Houttuynia cordata, Scutellaria baicalensis, orientalis leaves, Astragalus, Angelica dahurica, Ligustrum lucidum, Polygonum multiflorum, Dried ginger, Salvia miltiorrhiza, Dandelion, perilla leaf Sophora flavescens, licorice, loquat leaves, Chuanxiong, etc. The 18 flavors of Chinese herbal medicines were extracted by water, 60% ethanol or 95% ethanol, and the extracts were screened by dopa rate oxidation test, and the extracts of medicinal materials with strong activating effect on tyrosinase were obtained. Then the medicinal materials were combined to screen for enzyme activity.
[0026] Dopa rate oxidation test method:
[0027] 1.0 mg (1380 U) of tyrosinase was dissolved in 25 ml of PBS (0.05 mol / L, pH 6.8) buffer solution and prepared to a concentration of 55 U / ml. L-Dopa was prepared as a 0.15% solution in the above-mentioned PBS. The reaction system is 250 microliters in total, and the reagents are added in the following ta...
Embodiment 2
[0040] In vivo experiment: Three groups of medicinal material combinations with synergistic effects were obtained through in vitro enzyme activity screening. After a large number of preliminary drug efficacy evaluations in each group, it was found that adding other medicinal materials on the basis of the three combinations would increase the incidence of black hair. Efficacy, the effect of dried ginger and dandelion is more significant. Finally, 6 groups of compound formulas are designed for animal experiments. The specific prescriptions are shown in the table below:
[0041] Ratio of medicinal materials in 6 groups of experiments in table 1
[0042] Numbering Ligustrum He Shou Wu dried ginger Danshen dandelion Perilla leaves (water extraction) 1 0 30 0 30 10 30 2 30 0 30 10 0 30 3 10 30 0 30 0 30 4 20 20 10 20 10 20 5 20 20 20 0 10 30 6 20 20 10 30 10 0
[0043] experimental method:
[0044]...
Embodiment 3
[0046] Embodiment 3: the investigation of UFA effect
[0047] Experimental animals: C57 mice, male, weighing 18-20g, SPF grade, provided by Shanghai Xipuer-Bikay Animal Experiment Co., Ltd., animal production license number: SCXK (Shanghai) 2013-0016
[0048] Establishment of hydroquinone-induced gray hair mouse model:
[0049] On the first day of the experiment, mice were anesthetized by intraperitoneal injection of 4% chloral hydrate, weighed, and injected at 0.1ml / 10g body weight. After the mice were anesthetized, the long hair on the back was removed with an electric razor, and the process was slight to avoid damaging the skin. Then use a 1ml syringe to absorb 1ml of 6% sodium sulfide and apply it evenly on the back of the mouse. After 2.5 minutes, wash it off with warm water. At this time, the hair on the back of the mouse completely falls off and the skin is intact.
[0050] On the 7th day of the experiment, a 1ml syringe was used to absorb hydroquinone, and 0.5ml was ...
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