Method for simultaneously detecting six acidifying agents in composite acidifying agent

Abstract

The invention relates to a method for simultaneously detecting six acidifying agents in a composite acidifying agent, the six acidifying agents in the composite acidifying agent are respectively calcium formate, malic acid, lactic acid, acetic acid, fumaric acid and calcium propionate, and the method comprises the following steps: (1) preparing a series of mixed standard solutions and a to-be-detected sample solution; (2) under a preset chromatographic condition, injecting the series of mixed standard solutions in the step (1) into a high performance liquid chromatograph, carrying out chromatographic analysis so as to determine the nature of retention time, and drawing a standard curve of a corresponding acidifier; and (3) under preset chromatographic conditions, injecting the sample solution to be detected into a high performance liquid chromatograph for sample analysis, and quantitatively calculating the concentrations and contents of the six acidifiers in the sample solution. Under the preset chromatographic conditions, a chromatographic column is reversed-phase C18, and a mobile phase is 0.01 mol / L ammonium dihydrogen phosphate aqueous solution with the pH value of 2.4-2.6.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to high-performance liquid chromatography analysis and detection, in particular to a method for simultaneously detecting six acidifying agents in a compound acidifying agent. Background technique [0002] Composite acidifier is made by compounding two or more single acidifiers according to a certain ratio. It can be used together with several acids, or it can be compounded with acids and salts. Adding acidifiers to the feed can adjust the environment in the digestive tract, increase the activity of digestive enzymes, reduce the acidity of the feed system, adjust the microbial balance of the digestive tract, participate in the metabolism of the body, and enhance the immunity of the body. The compound acidulant product is not composed of a single acidulant. For better acidifying performance, several acidulants are combined in a suitable ratio to form a compound acidulan...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many detection methods for acidifying agents, the most common ones are colorimetry, fluorescence method, spectrophotometry, high performance liquid chromatography, ion exchange chromatography, capillary electrophoresis, gas chromatography, etc., but most of these detection methods It is for a single organic acid, or a specific FFAP column is required for the detection of multiple organic acids by gas phase (expensive, not widely applicable)

Therefore, when it is necessary to detect the acidulant components in the compound acidulant, it is necessary to use its own pretreatment technology and analysis method, and the detection is time-consuming, laborious, and expensive.

There are also reports on the detection of various organic acids by tandem mass spectrometry, but this kind of analytical instrument is expensive and not easy to be widely promoted in my country's feed industry

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