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Liquid fermentation culture medium and application thereof

A liquid culture medium and liquid fermentation technology, applied in the field of lactic acid bacteria culture medium, can solve the problems of high price, limited use, high production cost, etc.

Inactive Publication Date: 2021-05-11
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current Lactobacillus fermentation media use complex components, cumbersome configuration, and high prices, which make the production cost high and limit its use.

Method used

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  • Liquid fermentation culture medium and application thereof
  • Liquid fermentation culture medium and application thereof
  • Liquid fermentation culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Liquid fermentation medium, which comprises the following components by weight: 2 parts of molasses, 4 parts of corn steep liquor, KH 2 PO 4 0.1 part, 100 parts of water.

[0015] The configuration method of liquid fermentation medium is as follows:

[0016] Weigh each component in parts by weight, dissolve with 100 parts of distilled water, and sterilize at 115° C. for 20 minutes at natural pH (about 3.0).

[0017] The components are 2 parts of molasses, 4 parts of corn steep liquor, KH 2 PO 4 0.1 part.

[0018] Liquid fermentation medium for the fermentation of Lactobacillus brauchneri BL4:

[0019] (1) The frozen-preserved Lactobacillus Brucella BL4 was streaked and activated on the MRS plate, and a single colony was picked and inoculated in the liquid MRS medium, and cultured at 30°C and 200rpm for 24 hours to make the seed solution;

[0020] The said Lactobacillus buchneri (Lactobacillus buchneri) BL4, the strain was preserved on October 22, 2020 in the Gen...

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Abstract

The invention relates to the technical field of lactobacillus culture media. The invention provides a liquid fermentation culture medium which is prepared from, by weight, 2 parts of molasses, 4 parts of corn steep liquor and 0.1 part of KH2PO4. The culture medium provided by the invention is prepared from raw materials which are low in price, and is suitable for culturing various lactobacillus.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria culture medium. Background technique [0002] Lactic acid bacteria are beneficial microorganisms that can ferment sugars into lactic acid. They can colonize the intestines of humans and animals. They have the functions of maintaining the balance of intestinal flora and regulating the body's immunity. They are widely used in industry, agriculture, animal husbandry, food and medicine, etc. field has been widely used. In recent years, the addition of lactic acid bacteria to feed has attracted more and more attention in the livestock and poultry industry. It can improve the immunity of livestock and poultry, reduce or even replace the use of antibiotics, and at the same time improve the utilization rate of feed and improve meat quality. Therefore, lactic acid bacteria have broad prospects in the livestock and poultry industry. [0003] Lactobacillus is an important group of lactic acid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/225C12R1/24C12R1/25
CPCC12N1/20
Inventor 李少杰胡成成曹先贺孙宪昀张振颖
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI