Application of deferoxamine mesylate in preparation of medicine for treating otitis media
A technology for deferoxamine mesylate and otitis media, which is applied in the field of medicine, and can solve problems such as skin rash, diarrhea, otitis media protracted healing, and chronic otitis media.
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[0018] 1. OM mouse model construction and RPM treatment
[0019] Take 6–8 week-old C57BL / 6J mice (from Jackson Laboratory), measure their ABR threshold, select 25 mice with normal ABR threshold, including 10 female mice and 15 male mice, and divide them into Randomly divided into five groups.
[0020] The mice in each group were anesthetized with 4% chloral hydrate, and the following drugs were injected into the middle ear cavity of the mice in each group through the tympanic membrane using a Hamilton syringe: normal saline (NS), PGPS (100 μg), PGPS (100 μg)+DFO (100 μg ), PGPS (100 μg) + DFO (200 μg), PGPS (100 μg) + DFO (400 μg), the drugs were all dissolved in normal saline, and the injection volume was 10 μL.
[0021] 2. Auditory Brainstem Response (ABR)
[0022] The ABR threshold of C57BL / 6J mice was evaluated before and on the third day after PGPS injection:
[0023] Anesthetize the mouse with 4% chloral hydrate and keep its body temperature at 37°C; insert the active...
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