Immunohistochemical immune enzyme labeling method and kit for immune enzyme labeling method

An immunohistochemistry and immunoenzyme technology, applied in the biological field, can solve the problems of inconvenient use, unreasonable dosage and high production cost, etc.

Pending Publication Date: 2021-05-18
SUZHOU CANCERCELL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the current immunohistochemical method is complicated to operate, the staining effect is extremely unstable, the preparation method of each dye s

Method used

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Embodiment Construction

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0038] This embodiment relates to an immunoenzyme labeling method of immunohistochemistry, comprising the following steps:

[0039] Paraffin blank sections were placed in an oven at 60°C for 1 hour;

[0040] Dewaxing the baked slices in xylene, repeated 3 times, each time for 5 minutes;

[0041] Put the dewaxed slices in absolute ethanol for 1 min, in 95% ethanol for 30 s, in 75% ethanol for 30 s, soak in tap water for 1-2 min, and rinse with PBS for 3 times, each time for 3 min;

[0042] The dehydrated slices were immersed in 0.0...

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Abstract

The invention discloses an immunohistochemical immune enzyme labeling method, which mainly comprises the following steps of paraffin sectioning, xylene dewaxing, gradient alcohol dehydration, blocking, antigen repairing, sealing, primary antibody adding, secondary antibody adding, SP adding, developing, restaining and the like. The immunohistochemical immune enzyme labeling method is simple to operate, the color can be accurately controlled through microscopic examination, over-dyeing is avoided, and the dyeing effect is ensured. The preparation method of each solution is simple, the dosage is proper, the use is convenient, and waste is avoided. The invention further discloses a kit for the immune enzyme labeling method. The kit comprises an endogenous peroxidase blocking agent, a high-efficiency confining liquid, biotin-labeled goat anti-mouse IgG and streptomycete avidin-peroxidase. The kit comprises the solvents required by the immune enzyme labeling method, and each solvent is packaged by dropping bottles, so that the kit is convenient to use.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immunoenzyme labeling method of immunohistochemistry and a kit for the immunoenzyme labeling method. Background technique [0002] Immunohistochemistry is the application of the basic principle of immunology - antigen-antibody reaction, that is, the principle of specific binding of antigen and antibody, through chemical reaction to make the chromogenic agent (fluorescein, enzyme, metal ion, isotope) of the labeled antibody develop color. The determination of antigens (polypeptides and proteins) in tissue cells, their localization, qualitative and relative quantitative research, is called immunohistochemistry or immunocytochemistry. [0003] Immunohistochemistry has important functional significance for tumor pathological diagnosis and basic morphological research. This method is used to detect normal or abnormal products in cells or between cells, locate protein components, explo...

Claims

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Application Information

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IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 王守立
Owner SUZHOU CANCERCELL BIOTECH
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