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Application of dipeptide in increasing content of parastarch in euglena

A technology of paramylon and euglena, applied to single-celled algae, methods based on microorganisms, and the use of added compounds to stimulate growth, etc., can solve the problem of low paramylon content, achieve the effect of solving bottleneck problems and promoting research progress

Active Publication Date: 2021-05-28
YOUGE TIANCHENG BIOTECH YIWU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a technical problem that the content of paramylon in euglena is low in euglena cultivation at present.

Method used

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  • Application of dipeptide in increasing content of parastarch in euglena
  • Application of dipeptide in increasing content of parastarch in euglena
  • Application of dipeptide in increasing content of parastarch in euglena

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preparation example Construction

[0018] In the present invention, the preparation method of Glu-Ala comprises the following steps: the α-carboxyl group of Glu and the α-amino group of Ala are dehydrated and condensed to form an amide bond to obtain Glu-Ala.

[0019] In the present invention, the preparation method of Asp-Gly comprises the following steps: the α-carboxyl group of Asp and the α-amino group of Gly are dehydrated and condensed to form an amide bond to obtain Glu-Ala.

[0020] In the present invention, the preparation method of Arg-His comprises the following steps: the α-carboxyl group of Arg and the α-amino group of His are dehydrated and condensed to form an amide bond to obtain Glu-Ala.

[0021] In the present invention, the Glu-Ala, Asp-Gly and Arg-His were purchased from Bioengineering (Shanghai) Co., Ltd.

[0022] In the present invention, the application preferably includes the following steps:

[0023] 1) Carry out amplified culture to euglena until the OD of the algae liquid 750 When t...

Embodiment 1

[0045] 1. Source of algal species

[0046] The algal species used in the present invention: Euglena. gracilis CCAP 1224 / 5Z, purchased from CultureCollection of Algae and Protozoa.

[0047] 2. Culture medium composition

[0048] See Table 1 for common media formulations for euglena. Microelements a See Table 2 for the formula. The special medium for euglena is the usual medium for euglena plus 15g / L glucose.

[0049] 3. Algae cultivation method

[0050] Pick euglena from the euglena preservation plate and put it into a 150mL conical flask with 50mL of sterilized euglena common medium for activation. After it is cultivated to the logarithmic phase, transfer it to 1L of fresh euglena common medium solution for further expansion. Cultured, placed in a light incubator, cultured at 23±1°C, and the light intensity was 100±5μmol / m 2 the s 1 , continuous light. The whole operation process was carried out in accordance with the standard microbiological experimental method under ...

Embodiment 2

[0059] Except that Glu-Ala in Comparative Document 1 is replaced by Arg-His, the rest is the same as Example 1.

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Abstract

The invention provides application of dipeptide in increasing the content of parastarch in euglena, and belongs to the technical field of euglena culture. The dipeptide is selected from one or more of Glu-Ala, Asp-Gly and Arg-His. The dipeptide and euglena culture are utilized to promote the increase of the content of parastarch in euglena, the efficient output and sustainable culture of euglena biomass are realized, the research progress of the current euglena culture productivity mechanism can be promoted, and the bottleneck problem faced by the large-scale culture of euglena is solved.

Description

technical field [0001] The invention relates to the technical field of euglena cultivation, in particular to the application of dipeptide in increasing the paramylon content in euglena. Background technique [0002] Euglena gracilis is a unicellular eukaryotic microalgae. The cells have unique plant and animal features - flagellar movement and photosynthesis within the chloroplast can be observed in this organism. Since euglena produces valuable products such as wax esters, cloves and organic acids, it also contains nutritionally important all the essential vitamins and polyunsaturated fatty acids (docosahexaenoic acid and eicosapentaenoic acid acid). Euglena can utilize a variety of external carbon sources, such as glucose, ethanol, glutamic acid, malic acid, pyruvic acid, and lactic acid, for nutrient synthesis. Euglena cells can secrete an insoluble polysaccharide—Paramylon (Pm), whose main chain is β-1,3-linked glucose. Pm is a good immunostimulant and immune enhance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/38C12N1/12C12R1/89
CPCC12P19/04C12N1/38C12N1/12Y02A40/80
Inventor 贺嘉怡朱嘉怡刘永启秦换王立勇王江新
Owner YOUGE TIANCHENG BIOTECH YIWU CO LTD