Preparation method of novel rumen bypass glucose oxidase
A glucose oxidase and rumen-passing technology, which is applied in the field of preparation of new rumen-passing glucose oxidase, can solve the problem of less utilization of the small intestine, and achieve the goal of improving bioavailability, milk production, and dry matter intake Effect
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[0029] A preparation method of a novel rumen-passing glucose oxidase, comprising the steps of:
[0030] (1) Preparation of core material:
[0031] a. Mix glucose oxidase and hydrogenated oil according to the weight ratio of 1:6~9 to obtain mixture A for later use;
[0032] b. Mix the mixture A, flour, and pure water obtained in operation a in a weight ratio of 1:10~14:9~13 to obtain mixture B for later use;
[0033] c. Place the mixture B obtained in operation b in an electromagnetic field environment, and perform electromagnetic field-ultrasonic coupling treatment. The power of the electromagnetic field is controlled to 600~700W, the frequency of the ultrasonic wave is 20~24kHz, and the processing time is 5~9min. Extruded into 80~120 mesh pellets for later use;
[0034] d. Dry the pellets obtained in operation c in a low-temperature vacuum and store them in a low-temperature environment for later use. During drying, control the temperature at 32-36°C, vacuum degree at 1-2Pa...
Embodiment 1
[0043] A preparation method of a novel rumen-passing glucose oxidase, comprising the steps of:
[0044] (1) Preparation of core material:
[0045] a. Mix glucose oxidase and hydrogenated oil according to the weight ratio of 1:6 to obtain mixture A for later use;
[0046] b. Mix the mixture A, flour and pure water obtained in operation a in a weight ratio of 1:10:9 to obtain mixture B for later use;
[0047] c. Place the mixture B obtained in operation b in an electromagnetic field environment, and perform electromagnetic field-ultrasonic coupling treatment. Control the power of the electromagnetic field to 600W, the frequency of the ultrasonic wave to 20kHz, and the processing time to 5min. After completion, extrude into 80-mesh micro Pill spare;
[0048] d. Dry the pellets obtained in operation c in a low-temperature vacuum and store them in a low-temperature environment for later use. During the drying process, the temperature is controlled at 32°C, the vacuum degree is 1 ...
Embodiment 2
[0056] A preparation method of a novel rumen-passing glucose oxidase, comprising the steps of:
[0057] (1) Preparation of core material:
[0058] a. Mix glucose oxidase and hydrogenated oil according to the weight ratio of 1:7.5 to obtain mixture A for later use;
[0059] b. Mix the mixture A, flour and pure water obtained in operation a in a weight ratio of 1:12:11 to obtain mixture B for later use;
[0060] c. Place the mixture B obtained in operation b in an electromagnetic field environment, and perform electromagnetic field-ultrasonic coupling treatment. Control the power of the electromagnetic field to 650W, the frequency of the ultrasonic wave to 22kHz, and the processing time is 5 to 9 minutes. After completion, extrude it into 100 The purpose pellets are ready for use;
[0061] d. Dry the pellets obtained in operation c in a low-temperature vacuum and store them in a low-temperature environment for later use. During the drying process, the temperature is controlled...
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