Polypeptide co-modified naringin nanoliposomes for promoting proliferation and osteogenic differentiation of dental pulp stem cells, preparation method and application thereof

Abstract

The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to a polypeptide co-modified naringin nanoliposome for promoting the proliferation and osteogenic differentiation of dental pulp stem cells, its preparation method and application. The naringin nano liposome of the present invention is the naringin liposome prepared by co-modifying the RGD peptide and the TAT peptide. The polypeptide co-modified naringin nanoliposome prepared by the method of the present invention can significantly reduce the administration concentration, increase the uptake of the drug by cells, and promote the drug effect of naringin. Cell experiments have confirmed that the polypeptide co-modified naringin of the present invention On the premise that there is no obvious cytotoxicity and the safety of the preparation is fully guaranteed, the glycoside nanoliposome has shown a good effect on promoting the proliferation of dental pulp stem cells, and has an excellent ability to promote the osteogenic differentiation of dental pulp stem cells, so it has broad The development and clinical application prospects of relevant pharmaceutical preparations.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to a polypeptide co-modified naringin nanoliposome for promoting the proliferation and osteogenic differentiation of dental pulp stem cells, its preparation method and application. Background technique [0002] As a national treasure of our country, traditional Chinese medicine has obvious and definite curative effects in the treatment of various diseases. The traditional Chinese medicine Rhizoma Rhizoma Rhizoma Drynariae belongs to the kidney and liver meridian. It has the functions of nourishing the kidney and strengthening the bones, and relieving pain from injuries. It is one of the commonly used traditional Chinese medicines in the Department of Orthopedics and Traumatology. It is rich in active ingredients such as flavonoids, alkaloids, and phenols, among which naringin is one of its main active ingredients. Studies have found that naringin has...

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Problems solved by technology

Studies have found that naringin has antibacterial, anti-inflammatory, anti-viral, anti-cancer, anti-mutation, anti-allergic, anti-ulcer, analgesic, lowering blood pressure, lowering blood sugar and other activities, and can lower blood cholesterol and reduce the formation of thrombus , improve local microcirculation and nutrient supply, often used to prevent and treat food accumulation, abdominal distension, cough with phlegm, dysentery, diarrhea and cardiovascular and cerebrovascular diseases. At present, some scholars have conducted research on the role of naringin in promoting osteogenesis However, researchers have found that due to the poor water solubility of naringin, the cell uptake rate is low. In order to increase cell uptake and enhance drug efficacy, it is usually necessary to use a large amount of organic solvents and increase the dosage concentration in the process of cell and animal experiments, so that Greatly increased its cytotoxicity, which in turn caused great difficulties and risks to the development of related preparations and actual clinical applications, limiting its human application

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