Alternative template-imprinted polymers for specific recognition of exosomes and their applications
A technology of imprinting polymers and replacing templates, which is applied in the direction of microorganisms, biochemical equipment and methods, cell dissociation methods, etc., to achieve the effects of repeated use, wide application range, and easy operation
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Embodiment 1
[0025] Preparation of Imprinted Polymer (EXO-MIP1) Using Silica Nanoparticles as Alternative Templates
[0026] After mixing 50 mL of methanol and 7.74 mL of water, 5 mL of ammonia water with a mass concentration of 28% was added, and then 1.90 mL of tetraethyl silicate was rapidly added, and the reaction was stirred at 40° C. for 30 min. The particle size distribution was determined by dynamic light scattering (DLS) ( figure 1 ), and then centrifuged at 6000 g to obtain silica nanoparticles, which were washed three times with water and dispersed in 1 mL of water for later use.
[0027] Add 0.50 g of methylene bisacrylamide, 0.01 g of acrylamide, 0.01 g of 2-(diethylamino) ethyl acrylate and 0.10 g of silica nanoparticle dispersion into 6 mL of water. After dissolving and mixing, add 0.03g of azodiisobutylamidine hydrochloride initiates polymerization, reacts at 60°C for 24h, vacuum-dries at 60°C, grinds and sieves to obtain a polymer with a particle size of 10-50μm, and uses...
Embodiment 2
[0029] Preparation of Imprinted Polymer (EXO-MIP2) Using PLA-Glycolic Acid Nanoparticles as Alternative Templates
[0030] Add 0.02 g of aminopropyltriethoxysilane, 1 g of tetraethyl silicate, and 200 μL of an aqueous dispersion of polylactic acid-glycolic acid nanoparticles with a particle size distribution of 40-100 nm at a concentration of 2 g / mL into 10 mL of water , after dissolving and mixing, adding 0.04g of 28% ammonia water, reacting at 60°C for 24 hours, drying at 70°C, grinding, and sieving to obtain a polymer with a particle size of 10-50 μm, etched at 800°C at high temperature to remove polylactic acid - Glycolic acid nanoparticles, resulting in a surrogate template imprinted polymer (EXO-MIP2).
Embodiment 3
[0032] Preparation of Imprinted Polymer (EXO-MIP3) Using Polystyrene Nanoparticles as Alternative Templates
[0033] Add 0.04g of aminopropyltriethoxysilane, 1g of tetraethyl silicate and 100μL of polystyrene nanoparticle dispersion with a particle size distribution of 40-100nm at a concentration of 1g / mL into 8mL of water to dissolve After mixing, add 0.16g of 28% ammonia water, react at 50°C for 48h, dry at 50°C, grind and sieve to obtain a polymer with a particle size of 10-50μm, etch at 700°C for 12h to remove polystyrene nanoparticles, resulting in an alternative template imprinted polymer (EXO-MIP3).
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