Method for relieving phytotoxicity effect of atrazine on soybeans through inoculation of rhizobium

A technology of atrazine and rhizobia, which is applied in the field of agricultural microorganisms, can solve the problems of atrazine's severe phytotoxicity to soybeans, and achieve the purpose of alleviating soybean phytotoxicity, alleviating light damage and nutrient deficiency, and inhibiting growth The effect of tumor number

Pending Publication Date: 2021-06-11
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the technical problem that the residual atrazine in the existing black soil area has a serious phytot

Method used

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  • Method for relieving phytotoxicity effect of atrazine on soybeans through inoculation of rhizobium
  • Method for relieving phytotoxicity effect of atrazine on soybeans through inoculation of rhizobium
  • Method for relieving phytotoxicity effect of atrazine on soybeans through inoculation of rhizobium

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Experimental program
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Effect test

Example Embodiment

[0015] DETAILED DESCRIPTION OF THE INVENTION First: This embodiment is inoculating a method of relying on the role of Atlasi to soy medicines by Rhindroma, which is carried out in the following steps:

[0016] First, the rhizus strain is transferred to the YMA plane medium activation, and then the single collaboration is cultured in YMA liquid medium to obtain a nungal fluid;

[0017] Second, planting soybeans in the soil contaminated with Attra, when the soybean seedlings grow the first on the true leaf, injecting the rootenia solution described around the roots of soybean seedlings, continuing to cultivate, complete the method.

Example Embodiment

[0018] DETAILED DESCRIPTION OF THE INVENTION Different: The present embodiment differs from the specific embodiment: Step A root of the roots BRADYRHIZOBIUM JAPONICUM AC20, purchased from Shanghai Safe Biotechnology Center, and the preservation number is SHBCCD11101. Other as the specific embodiment.

Example Embodiment

[0019] DETAILED DESCRIPTION OF THE INVENTION Third: The present embodiment is different from that of the specific embodiment: the step of using a diluted or concentrated method to adjust the number of effective live bacteria in the root of the rootic macrobacteria to 1.2 × 10 7 ~ 1.2 × 10 9 CFU / ml. Others are the same as those of the specific embodiments.

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Abstract

The invention discloses a method for relieving the phytotoxicity effect of atrazine on soybeans through inoculation of rhizobium, and relates to the field of agricultural microorganism application. The invention aims to solve the technical problem that existing residual atrazine in the black soil area has a serious phytotoxicity effect on soybeans. The method comprises the following steps of activating and culturing the rhizobium strain to prepare a rhizobium solution; and planting soybean seeds, after each soybean seedling grow out a first pair of true leaves, the rhizobium solution in the first step is injected into the peripheries of the roots of the soybean seedlings, and cultivation continues. According to the method, after the rhizobium is applied, the stress effect of the atrazine on soybean growth can be obviously relieved. The method is used for relieving the phytotoxicity effect of the atrazine on the soybeans.

Description

technical field [0001] The invention relates to the application field of agricultural microorganisms. Background technique [0002] Corn and soybean rotation is a sustainable planting method widely used in Northeast my country, which can effectively maintain soil fertility. However, in actual production, the perennial continuous cropping of corn and the excessive application of herbicides lead to higher and higher residues of atrazine in the soil, and the damage to the next crop of soybeans is also more and more serious. Soybean is a kind of crop sensitive to atrazine stress. The nitrogen fixation system formed by legumes and root nodules is of great significance to the earth's ecosystem and agricultural production. In foreign countries, the biological nitrogen fixation function of soybeans has been fully utilized by inoculating rhizobia on a large scale, and obvious economic and ecological benefits have been achieved. However, nitrogen fertilizers are commonly used in soy...

Claims

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Application Information

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IPC IPC(8): A01G22/40A01G13/00
CPCA01G22/40A01G13/00
Inventor 张颖姜昭姜夺曹博邵琦安宁初玉欣聂亭元胡扬邓世杰
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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