A method for inhibiting the flourishing of cyanobacteria and a microalgae incubator
A technology of cyanobacteria and mixed culture, applied in the field of microalgae incubator, can solve problems such as difficult controllability, secondary pollution of water body, complex mechanism of biological and biological interaction, etc.
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Embodiment 1
[0069] The culture experiment was carried out with in situ water, that is: in a volume of 1m 3 Introduce in described incubator 10m in the Eighteen Bays of Taihu Lake 2 Close the lake water in the late winter and early spring in the enclosure until the water depth reaches 70cm, control the water temperature to be 15±1°C, add flocculant (chitosan:clay=1:10v / m), and use the agitator of the incubator 300 to water body Disturb for 30 minutes to make the algae in the water body flocculate and settle; after standing for 4 hours, let go of the supernatant and only keep the algae flocs at the bottom of the controller box, and then reintroduce the in-situ lake water that has filtered out the algae to a water depth of 70cm; Adjust the light intensity of the light fixture 500 in the incubator to 3000lx, and carry out the treatment of the light day cycle according to 12L:12D; open the top cover 400, and perform intermittent aeration, that is, the aeration tube 200 is aerated every two hou...
Embodiment 2
[0072] First, the mixed algae in the in-situ water body of Example 1 were classified, identified, tested and analyzed to obtain the dominant algae in the water body, and then the dominant single species of cyanobacteria and the dominant single species of algae-like algae were inoculated into the incubator for culture analysis; 1m 3 Add M11 enriched medium (100mg / L NaNO 3 +10mg / L K 2 HPO 4 +75mg / LMgSO 4 ·7H 2 O+40mg / L CaCl 2 ·H 2 O+20mg / L Na 2 CO 3 +6mg / L ferric citrate+1mg / L Na 2 EDTA·2H 2 O) to 70cm deep, with the same order of magnitude cell density (10 6 cells / L) Inoculate single species of cyanobacteria Microcystis aeruginosa (Microcystisaeruginosa), diatom Cyclotella hubeiana (Cyclotella hubeiana), green alga Quadrigulachodatii (Quadrigulachodatii) into the incubator box for mixed culture, and control the cultivation in the box The temperature of the system is 25±1°C, adjust the light intensity of the lamp 500 in the incubator to 3000lx, and carry out the treat...
experiment example 1
[0075] In order to study the influence of different culture temperatures on the growth of Microcystis aeruginosa, in this experiment example, a single species of Microcystis aeruginosa cell mother liquid was inoculated into M11 liquid medium for cultivation, and the light treatment conditions during the cultivation were controlled to 3000 lx, 12L:12D, set up three groups of experimental groups with different culture temperatures, i.e. 15°C group, 25°C group and 30°C group, culture for 24 days, observe the change of the cell density of Microcystis with temperature within 24 days, the results are as follows Figure 9 shown.
[0076] Depend on Figure 9 It can be seen that although the three temperature experimental groups all completed a round of life cycle within 24 days, the maximum cell density could be reached at 25°C, the growth rate was the fastest and the density peak was reached at the earliest at 30°C, and the cell density peaked at the earliest at 15°C. The slowest gr...
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