Method for stopping bleeding by mixing diatom shells with different structural characteristics
A technology with structural features and diatom frustules, which is applied in the field of hemostasis of mixed diatom frustules, can solve the problems of time-consuming and laborious, cumbersome modification process, and affect the hemostatic effect, etc., and achieve simple and easy operation process, simple operation process, and excellent hemostatic effect Effect
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Embodiment 1
[0042] 1 L of cultured Navicula sp. and Cocconeiopsis orthoneoides were packed into 100 mL centrifuge tubes and centrifuged at a centrifugal force of 2000 g for 5 minutes. After the centrifugation is completed, discard the supernatant, combine the diatom mud in each centrifuge tube, and wash with distilled water 3 times to remove inorganic salts and impurities. Add 25mL of concentrated sulfuric acid as a treatment solution to mix the algae cells with the concentrated sulfuric acid thoroughly, then put them in a 60°C water bath and heat for 45min. The heated mixture was placed at room temperature for 24 h. Add an equal volume of concentrated nitric acid to the mixture again, heat at 60°C until clear, and place at room temperature for 24h. The treated algal cells were collected by filtration, washed 5 times with distilled water and 5 times with absolute ethanol, and dried in an oven at 60°C. The collected white powder was diatom frustules. The diatom frustules of two kinds of ...
Embodiment 2
[0052] The cultured Navicula sp. and Cocconeiopsis orthoneoides were divided into 100 mL centrifuge tubes and centrifuged at a centrifugal force of 2000 g for 5 minutes. After the centrifugation is completed, discard the supernatant, combine the algae mud in each tube, and wash 3 times with distilled water. The acquisition and characterization methods of diatom frustules are the same as in Example 1.
[0053] The specific surface area is 120m 2 g -1 , the particle size is 10μm, the average pore size is 7.25μm, the shape index is 15, the liquid adsorption rate is 3010%, and the pore volume is 0.2202cc g -1 , Potential of -24.6mV Navicula shell and specific surface area of 190m 2 g -1 , the particle size is 22μm, the average pore size is 7.27μm, the shape index is 14, the liquid adsorption rate is 3500%, and the pore volume is 0.3475cc g -1 , Potential of -32.7mV and diatom frustules mixed fully at a ratio of 1:2 (M:M) to evaluate the hemostatic effect of mixed diatom f...
Embodiment 3
[0058] The cultured Navicula sp. and Cocconeiopsis orthoneoides algae fluids were divided into 100 mL centrifuge tubes for centrifugation with a centrifugal force of 2000 g and a centrifugation time of 5 minutes. After the centrifugation is completed, discard the supernatant, combine the algae mud in each tube, and wash 3 times with distilled water. The acquisition and characterization methods of diatom frustules are the same as in Example 1. The mixing method of diatom frustules was the same as that in Example 1, and the hemostatic effect in the femoral artery model of mixed algae frustules was evaluated.
[0059] The specific evaluation methods are:
[0060] 250-300g anesthetized male SD rats were fixed on the dissection table, and the skin of the right lower limb was cut with surgical scissors to expose the femoral artery; the femoral artery was cut open with a scalpel, and 50 mg of mixed diatom shells (Naviformis: egg Algae = 1:2), use the pre-weighed filter paper to abs...
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