Disposable bioreactor adopting cylindrical surface coupling overhead magnetic stirrer

Abstract

The invention discloses a disposable bioreactor adopting a cylindrical surface coupling overhead magnetic stirrer. The disposable bioreactor comprises a disposable biological reaction bag, a protective shell, an inner rotor, an outer rotor, an isolation sleeve, a stirring driving motor, a stirring shaft and stirring blades, wherein the isolation sleeve is arranged outside the inner rotor in a sleeving manner; the outer rotor is arranged outside the isolation sleeve in a sleeving manner; inner magnetic steel is arranged in the inner rotor; outer magnetic steel is arranged in the outer rotor; the outer rotor and the inner rotor are separated by the isolation sleeve; the bottom of the isolation sleeve is bonded and fixed with the top of the biological reaction bag to seal the bag opening; the upper part of the stirring shaft penetrates through the center of the inner rotor and is mounted in the center in the isolation sleeve through an upper-end bearing and a lower-end bearing; the stirring blades are arranged at the lower part of the stirring shaft; and a motor output shaft of the stirring driving motor is connected with the top end of the outer rotor. According to the disposable bioreactor, the stirring torque is higher, the bottom of the bag does not need to be supported, a stirring system does not need to be supported at the bottom, the height of the stirring blades can be adjusted according to the amount of a culture medium, and amplification culture in the same reactor can be achieved.

Description

technical field [0001] The invention belongs to the technical field of biological reaction equipment, and relates to a disposable bioreactor adopting a cylindrical surface coupling top-mounted magnetic stirrer. Background technique [0002] With the development of biological cell culture technology, stirred bioreactors are more and more widely used in biological cell culture. The traditional stirring bioreactor adopts stainless steel bioreactor; the existing top-mounted magnetic stirrer for stainless steel bioreactor includes: a housing, a driving device arranged on the housing, and a drive shaft arranged in the housing The driving rotor connected to the driving shaft, the driven rotor corresponding to the driving rotor; the driving rotor and the driven rotor are provided with magnetic steel; the driving rotor and the driven rotor are provided with a metal isolation cover; the stirring shaft and the driven rotor connection; the bearing seat supports and connects the stirrin...

AI Technical Summary

Problems solved by technology

[0009] 2. The fulcrum at the bottom of the bag, because of the bearing, is not only easy to generate wear debris, but also easy to get stuck (when there are solid particles in the culture solution)

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