Freezing preservation method of wood-rotting fungi
A technology of cryopreservation and wood-rot fungus, which is applied in botany equipment and methods, horticulture, plant cultivation, etc. It can solve the problems of reduced survival rate of bacteria, expansion of mycelium cells, low germination rate, etc., and improve the survival rate and germination rate, vigorous mycelial growth, and fast germination
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Embodiment 1
[0081] This example provides a freezing deposit method of a mushroom strain 7 Qihe 2 and a mushroom strain L808. It includes the steps:
[0082] The oak granules are the matrix, and the oak particles are formed into a diameter of 3-8 mm, a thickness of 2-5 mm, and perform pre-wet treatment and sterilization treatment; under aseptic conditions; The matrix particles were laptoplated in the PDA plate, and a mixed medium of wood chips and PDA was produced. (It should be noted that the oak particles are used as the water-proof matrix, and other particles such as apple wood can also be selected as the preservation matrix in other embodiments.
[0083] Then, according to multi-point inoculation, the bacterial body to be deposited is seeded on the mixed medium, and cultured in a culture tank of 25 ± 1 ° C, until the filament spreads to the inside of the wood chip particles, and forms mycelial particles. (The mushroom strain Qihe No. 2 and the mushroom strain L808 were cultured separately)...
Embodiment 2
[0088] This embodiment provides a freezing deposit method of a flat mushroom 3015. It includes the steps:
[0089] The oak granules are the matrix, and the oak particles are formed into a diameter of 3-8 mm, a thickness of 2-5 mm, and perform pre-wet treatment and sterilization treatment; under aseptic conditions; The matrix particles were laminated on the PDA plate (i.e., PDA plate), a mixed medium of wood chip and PDA was produced. (It should be noted that the oak particles are used as the water-proof matrix, and other particles such as apple wood can also be selected as the preservation matrix in other embodiments.
[0090] Then, according to multi-point inoculation, the bacterial body to be deposited is inoculated on the mixed medium and placed in an incubator of 25 ± 1 ° C, until the filament spreads to the interior of the wood chip particles, and forms mycelial particles.
[0091] Then, the bacterial particles (i.e., wood chip particles for long mycelial bodies) were placed ...
Embodiment 3
[0094] This embodiment provides a freezing preservation method of a mushroom. It includes the steps:
[0095] The oak granules are a water-saving matrix, and the oak particles are formed into a diameter of 3-8 mm, a sheet-like or a block-shaped plasmium having a thickness of 2-5 mm, and pre-wet treatment and sterilization treatment;
[0096] Making a PDA plate, a multi-point in PDA culture is used to cultivate 3 days in PDA medium, and the filament is placed, and germination forms 0.5 mm colonies;
[0097] Then, under sterile conditions, the chipper matrix was laid to the PDA plate (3 days of the mushroom figaus). (It should be noted that the oak particles are used as the water-proof matrix, and other particles such as apple wood can also be selected as the preservation matrix in other embodiments. The culture tank was placed in a secondary incubator in 23 ± 1 ° C, until the bacterial spread to the inside of the wood chip particles, forming mycelial particles.
[0098]Then, the ba...
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