Device and method for correcting drifting of optical tweezers by using laser reflection light spots

Abstract

The invention discloses a device and a method for correcting drifting of optical tweezers by utilizing laser reflection light spots. The device for correcting the drift of the optical tweezers by using the laser reflection light spots structurally comprises a light source light path, a sample light path, a camera light path and a processor, wherein the light source light path comprises a laser, a first polarization beam splitter, a beam splitting light path and a second polarization beam splitter; the sample light path comprises a first dichroscope, a sample table and a position sensitive detector; the camera light path comprises a camera, and the camera obtains the light passing through the sample table to shoot a reflection light spot and a reference ball of the reference light beam; and the processor is used for correcting the drift of the captured light beam according to the drift value. According to the device for correcting the drift of the optical tweezers by using the laser reflection light spots, the linear polarization continuous light is divided into the reference light beam and the capture light beam, and the reference light beam has the same position change as the capture light beam; and according to the position change of the reference ball and the position change of the reference light beam, the relative drift of the captured light beam and the sample table is corrected after a drift value is determined.

Description

technical field [0001] The invention belongs to a device and method for correcting drift of optical tweezers, and belongs to the field of optical tweezers, in particular to a device and method for correcting drift of optical tweezers by using laser reflection spots. Background technique [0002] Optical tweezers use the optical traps formed by focused beams to capture micro-nano-scale objects, and have been widely used in various applications including nuclease movement steps, pauses and backtracking, and protein / nucleic acid folding. in the study of biophysical problems. [0003] Traditional optical tweezers usually adopt a surface-coupled configuration. In the experiment, one end of the sample is connected to the surface of the cover glass, and the other end of the sample is connected to the polystyrene ball captured by the optical trap. In this configuration, the sample is connected to the surrounding environment through the cover glass, which is easily disturbed by envi...

AI Technical Summary

Problems solved by technology

Therefore, compared with the optical tweezers of the surface coupling configuration, the optical tweezers of the dual-beam configuration are more complicated, expensive, and more time-consuming in forming samples, and the overall experimental efficiency is lower.

[0006] Secondly, the current method of measuring the position of the fiducial mark on the su

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