Rapid propagation and standardized cultivation method for radix angelicae pubescentis
A cultivation method and solitary living technology, applied in the field of plant cultivation, can solve the problems that the invasion of diseases and insect pests and pesticide residues cannot be ruled out, the cost of solitary living planting is high, and the consumption of nutrients, etc., so as to eliminate the invasion of diseases and insect pests and pesticide residues, and reduce the cost of solitary planting , the effect of promoting cell proliferation speed
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0047] Materials: solitary living roots are used as explants, and the specific steps are as follows:
[0048] Step 1, selection and sterilization of explants: select the living root as the culture material, wash it with tap water, rinse it under running water for 12 minutes, place it in 75% alcohol for 5-10 seconds, and then treat it with 0.1% alcohol Mercurate disinfection for 8 minutes, rinse with sterile water and set aside;
[0049] Step 2, germination of sprouts: inoculate the sterilized sprouts on MS+6-BA0.8 mg / L+NAA0.3mg / L medium, the pH value is 6.2, and the culture temperature is 25-28°C. 10 hours of light per day, light intensity 1500LX, buds germinate 8-12 days after inoculation, and seedlings with leaves form in about 15 days;
[0050] Step 3, cluster bud induction and proliferation culture: Cut off the seedlings and transfer to MS+6-BA0.6mg / L+IAA0.5mg / L medium until cluster buds are formed, the pH value is 6.2, and the culture temperature is 25 -28°C, 13 hours o...
Embodiment 2
[0060] Materials: solitary living roots are used as explants, and the specific steps are as follows:
[0061] Step 1, selection and sterilization of explants: select the living root as the culture material, wash it with tap water, rinse it under running water for 12 minutes, place it in 75% alcohol for 5-10 seconds, and then treat it with 0.1% alcohol Mercurate disinfection for 6 minutes, rinse with sterile water and set aside;
[0062] Step 2, germination of sprouts: inoculate the sterilized sprouts on MS+6-BA0.8 mg / L+NAA0.3mg / L medium, the pH value is 6.2, and the culture temperature is 25-28°C. 10 hours of light per day, light intensity 1500LX, buds germinate 8-12 days after inoculation, and seedlings with leaves form in about 15 days;
[0063] Step 3, cluster bud induction and proliferation culture: Cut off the seedlings and transfer to MS+6-BA0.6mg / L+IAA0.5mg / L medium until cluster buds are formed, the pH value is 6.2, and the culture temperature is 25 -28°C, 13 hours o...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com