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A method for improving the ethanol yield of poplar synchronous saccharification and fermentation

A simultaneous saccharification and fermentation and ethanol technology, applied in fermentation, biofuels, fungi, etc., to achieve mild conditions, improve efficiency, and good application prospects

Active Publication Date: 2022-03-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strong acidic conditions generated by the addition of acid catalysts can remove most of the hemicellulose and lignin, but under severe pretreatment conditions, it can also lead to the degradation of cellulose or the degradation of xylose to furfural

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 A kind of method for improving ethanol production efficiency of poplar synchronous saccharification and fermentation

[0032] S1. Add 60% (v / v) ethanol aqueous solution to the poplar raw material according to the absolute dry mass volume ratio of 1g: 10mL, and then add 0.025mol / L 乙醇水溶液 AlCl 3 , reacted in the reactor at 200°C for 10 minutes, and centrifuged to obtain AlCl 3 Coupling ethanol pretreatment of poplar; mixing pretreated poplar with deionized water, so that the mass ratio of the absolute dry mass of pretreated poplar to deionized water is 12:100, and the pretreated poplar-water mixture is obtained;

[0033] S2. Add nutrient salts to the pretreated poplar wood-water mixture obtained in step S1, adjust the pH to 4.8, put it in an autoclave for sterilization at 121° C. for 20 minutes, and obtain the sterilized poplar wood mixture; wherein, the nutrient salt The amount added is 4.3g / L 去离子水 , yeast extract, NH 4 Cl, KH 2 PO 4 , MgSO 4 ·7H 2 Th...

Embodiment 2

[0035] Embodiment 2 A kind of method that improves the ethanol production efficiency of poplar synchronous saccharification and fermentation

[0036] S1. Add 50% (v / v) ethanol aqueous solution to the poplar raw material according to the absolute dry mass volume ratio of 1g: 8mL, and then add 0.03mol / L 乙醇水溶液 AlCl 3 , reacted in the reactor at 180°C for 20min, and centrifuged to obtain AlCl 3 Coupling ethanol pretreatment of poplar; mixing the pretreated poplar with deionized water, so that the mass ratio of the absolute dry mass of the pretreated poplar to the deionized water is 10:100, and the pretreated poplar-water mixture is obtained;

[0037]S2. Add nutrient salts to the pretreated poplar wood-water mixture obtained in step S1, adjust the pH to 4.5, put it in an autoclave for sterilization at 121° C. for 30 minutes, and obtain the sterilized poplar wood mixture; wherein, the nutrient salt The amount added is 2.1g / L 去离子水 , yeast extract, NH 4 Cl, KH 2 PO 4 , MgSO 4 ·...

Embodiment 3

[0039] Embodiment 3 A method for improving ethanol production efficiency by synchronous saccharification and fermentation of poplar

[0040] S1. Add 70% (v / v) ethanol aqueous solution to the poplar raw material according to the absolute dry mass volume ratio of 1g: 15mL, and then add 0.01mol / L 乙醇水溶液 AlCl 3 , reacted in the reactor at 200°C for 5 minutes, and separated by vacuum filtration to obtain AlCl 3 Coupling ethanol pretreatment of poplar; mixing pretreated poplar with deionized water, so that the mass ratio of the absolute dry mass of pretreated poplar to deionized water is 13:100, and the pretreated poplar-water mixture is obtained;

[0041] S2. Add nutrient salts to the pretreated poplar wood-water mixture obtained in step S1, adjust the pH to 6.0, put it in an autoclave for sterilization at 121° C. for 20 minutes, and obtain the sterilized poplar wood mixture; wherein, the nutrient salt The amount added is 6.5g / L 去离子水 , yeast extract, NH 4 Cl, KH 2 PO 4 , MgSO ...

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Abstract

The invention provides a method for increasing the ethanol yield of poplar synchronous saccharification and fermentation. The present invention combines poplar raw material with AlCl 3 Mix ethanol water solution, react at 180-200°C for 5-20 minutes for pretreatment, then add water to adjust the solid load of pretreated poplar to 10-13%, add nutrient salts, add cellulase and yeast after sterilization Simultaneous saccharification and fermentation. The present invention has mild pretreatment conditions, can effectively remove hemicellulose and lignin in poplar, retain cellulose, and carry out synchronous saccharification and fermentation for 48 hours, and the ethanol concentration in the fermentation liquid is higher than 30g / L, which can be greatly improved in a short time The efficiency of ethanol production by fermentation has a good application prospect in the preparation of liquid fuel ethanol from poplar.

Description

technical field [0001] The invention belongs to the technical field of biomass transformation and utilization, and more specifically relates to a method for increasing the ethanol yield of poplar synchronous saccharification and fermentation. Background technique [0002] As energy shortages and environmental issues become increasingly prominent, biomass, as a renewable resource that can be converted into liquid fuels, is receiving increasing attention. Among the abundant biomass resources on the earth, the production of liquid fuels from lignocellulosic biomass has attracted the most attention. The method of producing liquid fuel from lignocellulosic biomass is to hydrolyze hemicellulose and cellulose in the raw material into monosaccharides such as xylose and glucose, and produce ethanol through fermentation. [0003] Poplar is rich in resources and is an important raw material for the production of bioenergy and biomass chemicals. Biotransformation of poplar wood to prod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14C12P19/02C12N1/16
CPCC12P7/10C12P19/14C12P19/02C12N1/16C12P2201/00Y02E50/10
Inventor 张红丹黄心宇谢君
Owner SOUTH CHINA AGRI UNIV