Extraction method for cistanche lanzhouensis polysaccharide and optimization method thereof

A Cistanche polysaccharide and extraction method technology, applied in the field of extraction and optimization of Cistanche polysaccharides in Lanzhou, can solve the problems of shortening the extraction time, polysaccharide structure changes, and ultrasonic extraction time should not be too long, so as to improve the extraction rate and save extraction time. Effect

Pending Publication Date: 2021-07-16
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST +1
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Problems solved by technology

Compared with water extraction and alcohol precipitation method, it greatly shortens the extraction time and does not change the structure of polysaccharides. It has been widely used in the extraction of polysaccharides i

Method used

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  • Extraction method for cistanche lanzhouensis polysaccharide and optimization method thereof
  • Extraction method for cistanche lanzhouensis polysaccharide and optimization method thereof
  • Extraction method for cistanche lanzhouensis polysaccharide and optimization method thereof

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Example Embodiment

[0031] Example 1

[0032]A method of extraction method of Lanzhou Cistanche polysaccharide, which is a microwave-ultrasonic synergistic method. It is specifically to extract its polysaccharide after Lanzhou Cistanche Sample. After centrifugal treatment, the microwave-ultrasonic synergistic method is performed, of which the microwave-ultrasonic synergistic law The extraction conditions are: from 1:40, ultrasonic power 1000W, microwave power 500W, extraction time is 8 min, and the number of times is 3 times.

[0033] Among them, the specific steps include the following:

[0034] Step 1, the samples use Lanzhou meat raft fleshy stems, from Kyushu District, Lanzhou, refrigeration, spare after fresh sample collection;

[0035] Step 2, the step 1 is frozen, the meat stems are frozen, clean, dry and pulverize, take the powder to add fresh and extract, centrifugally, to remove filter residue, spare; where ethanol reflow degreasing is: will crush Lanzhou 700 ml, 850ml and 1000 ml of 95% et...

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Abstract

The invention relates to the technical field of cistanche deserticola polysaccharide extraction, in particular to an extraction method for cistanche lanzhouensis polysaccharide and an optimization method thereof. The extraction method is a microwave-ultrasonic wave synergistic method, and specifically comprises the following steps: degreasing and centrifuging a cistanche lanzhouensis sample, and extracting the polysaccharide of the cistanche lanzhouensis by using a microwave-ultrasonic wave synergistic method under the extraction conditions that a material-liquid ratio is 1: 40, ultrasonic power is 1000 W, microwave power is 500 W, extraction time is 8 min, and an extraction frequency is 3. According to the extraction method disclosed by the invention, the actual yield of the cistanche lanzhouensis polysaccharide is measured to be 51.84%. According to the optimization method, the actual value of a regression model is close to a theoretical value, which shows that the regression model can well reflect the influence of the material-liquid ratio, the ultrasonic power and the microwave power on the yield of the cistanche lanzhouensis polysaccharide, and the method is proved to be feasible in extracting the cistanche lanzhouensis polysaccharide. From the aspect of the extraction time of optimized parameters, the extraction time can be greatly saved and the extraction rate of the polysaccharide can be greatly improved when the cistanche lanzhouensis polysaccharide is extracted by using the microwave-ultrasonic wave synergistic method.

Description

technical field [0001] The invention relates to the technical field of extracting polysaccharides from Cistanche deserticola, in particular to an extraction and optimization method of polysaccharides from Cistanche deserticola in Lanzhou. Background technique [0002] Cistanche deserticola (Cistanche deserticola Ma), also known as Jiangyun, Dayun (Dandayun), Cunyun, Cistanche (Cistanche, Cistanche), Goblin, Chagan Gaoya (Mongolian), Liedangke, Cistanche belongs to. Cistanche deserticola is a parasitic plant that lives on the root of the desert tree Haloxylon, absorbing nutrients and water from the Haloxylon host. Known as "Desert Ginseng", also known as "Desert Cistanche", it has extremely high medicinal value and is a traditional Chinese medicinal material. Cistanche has been regarded as a treasure by the Western Regions as a tribute to the imperial court in history, and it is also one of the most frequently used tonic drugs in the prescriptions for tonifying the kidney a...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 周玉碧王文舒王劼孟和巴雅尔郭俏俪马扎雅泰武志博钱鹏侯建华刘雪娟时玮郝佳辰马超刘玉珍李明聪
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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