Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aphidius gifuensis SSR markers and application thereof

The invention is a technology of M. philippines and labeling, which is applied in the field of molecular biology and can solve the problems of long-term reproduction, breeding and application of unfavorable M. philippines, genetic evaluation of the impact of wild populations, reduction of genetic diversity, etc., and achieves good application value, PCR The effect of stable amplification results and large number of alleles

Active Publication Date: 2021-07-16
CHINA AGRI UNIV +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the impact of the release of captive-bred Aphids persicae on wild populations has never been genetically assessed.
However, artificially bred populations often have reduced genetic diversity due to factors such as limited parents and inbreeding, which is not conducive to the long-term reproduction, breeding and application of Aphidius persicae.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aphidius gifuensis SSR markers and application thereof
  • Aphidius gifuensis SSR markers and application thereof
  • Aphidius gifuensis SSR markers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Acquisition of SSR markers and primers for Aphidius aphidis

[0052] In the present invention, 21 microsatellite-specific primers are selected for synthesis and screening. After validity and polymorphism testing, 16 specific microsatellite primers were finally obtained. Among them, the polymorphism of site S6 was low, and sites S12, S20, S29, and S35 deviated from the Hardy-Weinberg equilibrium, which would cause deviations in the subsequent genetic analysis of the Aphidius aphidense population, and would have a certain impact on the results.

[0053] 1. Sources of materials for Aphids aphids

[0054] Preliminary screening of the effective primers for Aphids persicae, using 8 representative samples from different regions, namely, Qianxi Fanfeng Factory in Bijie, Guizhou, Mile Dongfeng Farm in Honghe Prefecture, Yunnan, Linquan Town, Qianxi, Bijie, Guizhou, and Mudanjiang City, Heilongjiang Fanfeng Factory in Zhangjia Village, Ning'an Town, Ning'an City, Hedo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a group of aphidius gifuensis SSR markers and application thereof. The Aphidius gifuensis SSR markers comprise 16 SSR markers, the serial numbers of the 16 SSR markers are S1, S3, S5, S8, S10, S13, S15, S18, S30, S36, S38, S39, S40, S41, S42 and S44 respectively, and primers for amplifying the SSR markers are shown as SEQ ID NO: 1-32 respectively. According to the method, DNA is extracted from aphidius gifuensis samples from different regions, and microsatellite sites are screened, so that primers are designed; the group of primers has the advantages of stable PCR amplification result, high polymorphism and the like, can be used for analyzing the diversity and population genetic structure of aphidius gifuensis, and has good application value.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to the SSR marker of Aphidius aphidis and its application. Background technique [0002] Aphidius gifuensis Ashmead belongs to the genus Aphidius of the family Aphidiidae of the order Hymenoptera. It is distributed in Japan, Korea and the Hawaiian Islands in eastern Asia abroad; it is distributed in Jilin and Liaoning in China. , Shaanxi, Shandong, Henan, Jiangsu, Anhui, Zhejiang, Fujian, Taiwan, Guangdong, Yunnan and other provinces, mainly in the eastern and southern provinces of my country, are the dominant natural enemies of aphids. In addition to parasitizing Myzus persicae (Sulzer), Aphids persicae can also parasitize vegetable aphids Brevicoryne brassicae (Linnaeus), radish aphid Lipaphiserysimi (Kaltenbach), wheat long tube aphid Macrosiphum avenae (Fabricius), etc. The natural parasitism of aphids The rate is usually 20% to 60%, and the highest can rea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02A40/81
Inventor 李虎冯增贝杨海林周文斌张立猛彩万志
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com