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Method for producing wheat oligopeptide by precipitation and application

A technology of wheat oligopeptide and precipitation method, which is applied in the preparation method of peptide, chemical instruments and methods, peptide, etc., which can solve the requirements of increasing equipment cost and production cost, affecting product quality, feed liquid dry basis and suspended solids Higher problems, to achieve the effect of saving production time, improving efficiency and reducing investment

Active Publication Date: 2021-07-23
JIANGSU ZHIHUI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention provides a method and application for producing wheat oligopeptides by precipitation method, one of the purposes is to have the characteristics of separation by sedimentation method according to the physical and chemical characteristics of feed liquid, and solve the problem of centrifugal separation process and membrane filtration. The process has high requirements on the dry basis of the feed liquid and suspended solids; another purpose is to have a method of controlling the concentration of the feed liquid, the degree of enzymatic hydrolysis and promoting natural precipitation, so that the feed liquid can be left standing to separate a large amount of supernatant Liquid, the extracted clear supernatant after membrane filtration and drying is the characteristic of qualified wheat oligopeptide products. It solves the need for secondary separation in the centrifugal separation process of enzymatic hydrolysis liquid, and the separation equipment has strict requirements on the content of suspended solids in enzymatic hydrolysis liquid. Another purpose is to have the characteristics of not adding additives to promote enzymolysis and separation effects in the enzymolysis process, and to solve the problem of adding substances to promote enzymolysis and separation in the pulping and enzymolysis processes. Filtration process needs to continue nanofiltration for deashing treatment, which increases equipment cost and production cost, and affects product quality

Method used

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  • Method for producing wheat oligopeptide by precipitation and application
  • Method for producing wheat oligopeptide by precipitation and application
  • Method for producing wheat oligopeptide by precipitation and application

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Experimental program
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Embodiment 1

[0036] like figure 1 As shown, the present invention provides a method and application of producing wheat oligopeptides by precipitation method, the method and application of producing wheat oligopeptides by precipitation method, comprising the following steps:

[0037] S1, take gluten powder slurry: take 980kg gluten powder, mix with 3500 kilograms of 20 ℃-40 ℃ warm water in reaction tank, be mixed with the slurry that dry basis concentration is about 20%;

[0038] S2, enzymatic hydrolysis;

[0039] S2.1. Adjust the pH of the feed liquid in S1 to 7.5-8.0, add 14.7 kg of Alcalase2.4L protease with a material ratio of 1.5%, mix and stir, and enzymolyze at 50-60° C. for 4 hours;

[0040] S2.2. Adjust the pH of the feed liquid in S2.1 to 6.8-7.2, add 1% Neutrase 0.8L protease and 0.1% Flavourzyme 500MG flavor protease, and enzymatically hydrolyze at 50-60 degrees for 2 hours;

[0041] S2.3. Sampling the feed liquid in S2.2, and measuring the molecular weight distribution with h...

Embodiment 2

[0052] like figure 1 Shown, on the basis of embodiment 1, the present invention provides a kind of technical scheme: a kind of method and application of producing wheat oligopeptide with precipitation method, the method and application of this production wheat oligopeptide with precipitation method, comprise the following step:

[0053] S1, take gluten powder slurry: take 980kg gluten powder, mix with 3500 kilograms of 20 ℃-40 ℃ warm water in reaction tank, be mixed with the slurry that dry basis concentration is about 20%;

[0054] S2, enzymatic hydrolysis;

[0055] S2.1. Adjust the pH of the feed solution in S1 to 7.5-8.0, add 14.7kg of Alcalase2.4L protease with a material ratio of 1.0%, mix and stir, and enzymolyze at 50-60°C for 4 hours;

[0056] S2.2. Adjust the pH of the feed solution in S2.1 to 6.8-7.2, add 1.5% Neutrase 0.8L protease and 0.1% Flavourzyme 500MG flavor protease, and enzymatically hydrolyze at 50-60 degrees for 2 hours;

[0057] S2.3. Sampling the fee...

Embodiment 3

[0068] like figure 1 Shown, on the basis of embodiment 1, the present invention provides a kind of technical scheme: a kind of method and application of producing wheat oligopeptide with precipitation method, the method and application of this production wheat oligopeptide with precipitation method, comprise the following step:

[0069] S1, take gluten powder slurry: take 980kg gluten powder, mix with 3500 kilograms of 20 ℃-40 ℃ warm water in reaction tank, be mixed with the slurry that dry basis concentration is about 20%;

[0070] S2, enzymatic hydrolysis;

[0071] S2.1. Adjust the pH of the feed solution in S1 to 7.5-8.0, add 14.7 kg of Alcalase2.4L protease with a material ratio of 1.5%, mix and stir, and enzymolyze at 50-60°C for 2 hours;

[0072] S2.2, then adjust the pH of the feed solution in S2.1 to 6.8-7.2, add 1% Neutrase 0.8L protease and 0.1% Flavourzyme 500MG flavor protease, and enzymatically hydrolyze at 50-60 degrees for 2 hours;

[0073] S2.3. Sampling the...

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Abstract

The invention discloses a method for producing wheat oligopeptide by precipitation and application, and relates to the technical field of wheat oligopeptide production. The method for producing wheat oligopeptide by precipitation and the application comprise the following steps: S1, taking gluten flour for pulping; S2, performing enzymolysis; S3, extracting supernate: standing the material liquid in the S2 for 2-4 hours, and starting to extract the supernate when the proportion of the supernate reaches 60%-70%; and S4, performing enzymolysis on the supernate again. The invention provides the method for producing wheat oligopeptide by precipitation and the application, centrifugal separation equipment and complex membrane filtration equipment are not needed in the production process, separation is carried out by a sedimentation method according to the physical and chemical characteristics of the material liquid, the membrane filtration equipment is simple and efficient membrane filtration equipment, the product quality meets the quality requirement of wheat oligopeptide new resource food, the problems of complex production process flow, large production equipment investment and high production cost are avoided, the flow of the production process is simplified, and the investment of the production equipment is reduced.

Description

technical field [0001] The invention relates to a method for producing wheat oligopeptides, relates to the technical field of wheat oligopeptide production, in particular to a method and application for producing wheat oligopeptides by a precipitation method. Background technique [0002] Wheat oligopeptides are made from wheat gluten through pulping, protease hydrolysis, separation, filtration, spray drying and other processes. / (g / 100g)≥90, oligopeptide (dry basis) / (g / 100g)≥75, total glutamic acid / (g / 100g)≥25, protein hydrolyzate with relative molecular weight less than 1000 Proportion (%) ≥ 85, moisture / (g / 100g) ≤ 7, ash content / (g / 100g) ≤ 7, one of the characteristics of wheat oligopeptides is high glutamine, which can effectively regulate nerves and can also be used as intestinal Special nutrients for dysfunction. There are following problems at prior art: [0003] The production process of traditional wheat oligopeptide powder is: gluten flour mixing, enzymatic hydr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC12P21/06C07K1/34C07K1/14
Inventor 胡竑邠李宝林高卫东成廷水张翼
Owner JIANGSU ZHIHUI BIOTECH