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Preparation of porcine reproductive and respiratory syndrome and Mycoplasma hyopneumoniae dual-polyepitope vaccine

A technology for Mycoplasma hyopneumoniae and respiratory disorder, which is applied in multivalent vaccines, vaccines, medical preparations containing active ingredients, etc., can solve problems such as cross-infection, vaccine protection effect, economic loss, etc., and achieves high safety and PRRSV prevention. and Mhp infection, cost saving effect

Active Publication Date: 2022-08-02
HEBEI NORMAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

Porcine reproductive and respiratory syndrome virus and mycoplasma hyopneumoniae are two common pathogens in the pig industry. They are often cross-infected and cause huge economic losses. Potency will be affected

Method used

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  • Preparation of porcine reproductive and respiratory syndrome and Mycoplasma hyopneumoniae dual-polyepitope vaccine
  • Preparation of porcine reproductive and respiratory syndrome and Mycoplasma hyopneumoniae dual-polyepitope vaccine
  • Preparation of porcine reproductive and respiratory syndrome and Mycoplasma hyopneumoniae dual-polyepitope vaccine

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Embodiment Construction

[0049] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments.

[0050] like Figure 1-13B As shown, the preparation of porcine reproductive and respiratory syndrome and Mycoplasma hyopneumoniae dual polyepitope vaccine includes the following steps:

[0051] A. The antigenic epitopes of the GP5 protein and GP6 protein of PRRSV from the QHD strain: obtain the GP5 and GP6 protein epitope data of PRRSV, screen according to the location and length of the epitope, and screen out the corresponding PRRSV antigenic epitope PRRSV -GP5 and PRRSV-GP6;

[0052] B. Epitopes of P46 and P65 of Mhp: Use Bepipred Linear Epitope Prediction to predict B-cell epitopes of Mhp-P46 and Mhp-P65 proteins, and use ProPred-I to p...

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Abstract

The invention provides the preparation of porcine reproductive and respiratory syndrome and Mycoplasma hyopneumoniae dual-polyepitope vaccine, comprising the following steps: A, the antigenic epitopes of the GP5 protein and GP6 protein of PRRSV; B, the P46 and P65 epitopes of Mhp Epitope; C, Recombination of PRRSV and Mhp epitopes; D. Hydrophilic analysis and secondary structure analysis of recombinant epitope protein RP1; F. Construction of prokaryotic expression vector of recombinant epitope protein RP1; G. Expression and verification of recombinant antigenic epitope protein RP1; H. Purification and verification of recombinant antigenic epitope protein RP1. The invention can effectively prevent PRRSV and Mhp infection at the same time, greatly reduces the difficulty of operation, and saves the cost of the vaccine itself and manpower injection; it can prevent multiple diseases by one injection, and is suitable for the prevention of PRRSV and Mhp in the pig industry. High safety and good immune effect.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, and particularly relates to the preparation of a porcine reproductive and respiratory disorder syndrome and a Mycoplasma hyopneumoniae doublet polyepitope vaccine. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is a typical infectious disease characterized by reproductive failure of sows and respiratory distress in growing pigs. The pathogen is porcine reproductive and respiratory syndrome virus (PRRSV). ), with the ability of continuous infection and antigenic change, rapid evolution, strong environmental adaptability, and rapid spread, covering a wide range, which seriously restricts the healthy and sustainable development of the world pig industry. Mycoplasma hyopneumonia (Mhp) can cause swine panting disease, which is difficult to cure and often breaks out seasonally. Porcin respiratory disease complex (PRDC). [0003] Pig herds are often infe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/02A61K39/12A61P31/14C07K14/08C07K14/30C12N15/70
CPCC07K14/005C07K14/30C12N15/70A61K39/12A61K39/0241A61K39/39A61P31/04A61P31/14C12N2770/10022C12N2770/10034A61K2039/70
Inventor 张冉高志嵩赵宝华陈令孝潘璇李晓静路广宗
Owner HEBEI NORMAL UNIV