Aspergillus niger and application and method thereof
A technology of Aspergillus niger and processing methods, applied in biochemical equipment and methods, methods based on microorganisms, fungi, etc., can solve the problems of poor quality of dark tea, achieve low cost, improve color, and improve the quality of dark tea.
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experiment example 1
[0020] The separation and purification of experimental example 1 Aspergillus niger PW-2
[0021] Aspergillus niger PW-2 is isolated and purified in the following way:
[0022] Take tea leaves or tea stalks from different parts of Pingwufu tea bricks and inoculate them on PDA medium, and culture them at a constant temperature at 28°C. The strain.
[0023] The colony isolated above is Aspergillus niger, which can secrete a variety of extracellular enzymes, which can promote the degradation of proteins in tea, and can catalyze the oxidation of polyphenolic compounds to convert them into substances beneficial to the human body, improving the taste and efficacy of tea soup .
[0024] The Aspergillus Niger PW-2 (Aspergillus Niger PW-2) was deposited on October 21, 2020 at the China Center for Type Culture Collection located at Wuhan University, China, with the preservation number CCTCC NO: M 2020618.
experiment example 2
[0025] Morphological observation of experimental example 2 Aspergillus niger PW-2
[0026] Morphological observation of Aspergillus niger PW-2 of the present invention, specifically, the isolated Aspergillus niger PW-2 was cultured in PDA medium, MEA medium, CDA medium and CYA medium by single point inoculation method 7d (constant temperature cultivation under the condition of 25° C.) and observe the growth. Wherein, the preparation method of the PDA medium is: get 3g of potato powder, 20g of glucose and 14g of agar and mix them with 1L of water. The preparation method of the MEA medium is as follows: mix 130 g of malt extract powder, 0.1 g of chloramphenicol and 15 g of agar with 1 L of water, and adjust the pH of the culture system to 6.0±0.2. The preparation method of CDA culture medium is: get 3g sodium nitrate, 1g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 0.5g potassium chloride, 0.01g ferrous sulfate, 30g sucrose and 15g agar and mix with 1L water. The pr...
experiment example 3
[0034] Molecular biological identification of experimental example 3 Aspergillus niger PW-2
[0035] Carry out ITS molecular biology identification to Aspergillus niger PW-2 strain, specifically include the following steps:
[0036] Primers ITS1 and ITS4 were selected for amplification, the products were detected by agarose gel electrophoresis, and photographed by an ultraviolet imaging system; the sequence of the purified PCR products was determined, compared with the homology in the NCBI database, and a higher homology was selected The sequence was analyzed by using the MEGA analysis software, and the phylogenetic tree was constructed. The results are as follows image 3 shown.
[0037] The nucleotide sequence of the above primer ITS1 is as follows:
[0038] 5'-TCCGTAGGTGAACCTGCGG-3' (SEQ ID NO.1);
[0039] The nucleotide sequence of the above primer ITS4 is as follows:
[0040] 5'-TCCTCCGCTTATTGATATGC-3' (SEQ ID NO. 2).
[0041] Aspergillus niger PW-2 strain had the hi...
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