Application of paclitaxel combined with vinpocetine in preparation of medicine for treating lung cancer
A combined drug and paclitaxel technology, which is applied in the field of medicine, can solve the problems that have not yet been reported, and achieve the effect of improving anti-tumor effect and reducing toxic and side effects
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Embodiment 1
[0017] Embodiment 1 mouse pdx model is established
[0018] 1. Experimental materials
[0019] Matrigel® Matrix matrigel (Corning, USA), RPMI 1640 medium (Biological Industries, Israel), lung cancer human tumor tissue.
[0020] 2. Tumor tissue inoculation
[0021] 1. Thaw the Matrigel and mix it with 1640 medium at a ratio of 1:1 to dilute it, and put it on ice for later use.
[0022] 2. Dissect the human-derived tumor that has grown to a sufficient volume under the skin of nude mice and place it in a petri dish. Place the dish on ice and pour an appropriate amount of pre-cooled physiological saline.
[0023] 3. Carefully remove the necrotic part of the tumor tissue, blood vessels and tumor surface capsule, etc.
[0024] 4. Cut tumor tissue into 1 mm 3 Small pieces of about volume are mixed with diluted Matrigel for later use.
[0025] 5. Using a trocar, implant the small tumor mass mixed with Matrigel subcutaneously in the right side of the nude mouse.
[0026] 6. After...
Embodiment 2
[0027] Example 2 Changes in volume of transplanted tumor in mice
[0028] 1. Experimental group
[0029] 1. Blank group: no administration.
[0030] 2. Paclitaxel group: Paclitaxel 5 mg / kg was administered intravenously once.
[0031] 3. Vinpocetine group: Vinpocetine 3 mg / kg, intraperitoneal injection once.
[0032] 4. Combined drug group: paclitaxel administered by intravenous injection, 5 mg / kg, once; vinpocetine administered by intraperitoneal injection, 3 mg / kg, once.
[0033] 2. Results Analysis
[0034] The diameter of the tumor was measured before the administration started, and the diameter of the tumor was measured every 2 days thereafter until the end of the experiment. The mice were sacrificed by devertebral dissection the next day after the administration, and the subcutaneous tumor tissues were dissected and photographed.
[0035] Table 1 Changes in the volume of transplanted tumors in mice in each group
[0036] group Day 0 (mm 3 )
[0037] a...
Embodiment 3
[0038] Example 3 Tumor pathological HE staining
[0039] 1. HE staining
[0040] The dissected subcutaneous tumor tissues were fixed with fixative and prepared into paraffin sections. Dewax with xylene I, II and III for 3 minutes each, ethanol I and II for 2 minutes each, 95% ethanol, 90% ethanol, and 80% ethanol for 1 minute each, stain with hematoxylin for 10 minutes, and rinse with water 1% hydrochloric acid alcohol differentiation for 15 seconds, soaking in water for 10 minutes, eosin staining for 3 minutes, 80% ethanol and 90 ethanol for 10 seconds each, 95% ethanol for 1-2 minutes, absolute ethanol I, II, III for 3 minutes each, two Toluene I, II, III each for 3 minutes, and finally neutral gum sealing, photographed under a microscope.
[0041] 2. Results Analysis
[0042] according to figure 2 The displayed staining results show that the nuclear staining of the tumor cells in the blank group, the paclitaxel group and the vinpocetine group is obviously darker, the n...
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