A vascularized bone biomimetic multifunctional tissue engineering scaffold with anti-inflammatory effect and preparation method thereof
A technology for tissue engineering scaffolds and anti-inflammatory effects, applied in tissue regeneration, pharmaceutical formulations, prostheses, etc., to achieve anti-inflammatory preparation methods, good comprehensive performance, and the effect of promoting vascularization
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Embodiment 1
[0055] (2) Using FDM technology, biomimetic PLA scaffolds with gradient pore sizes of 500 μm and 900 μm were prepared by 3D printers. (3) At room temperature, the PLA scaffolds were soaked in ammonia solution with a pH value of 12 and stirred at a speed of 180 rpm for 4 h, and then the alkali-treated PLA scaffolds were soaked in a concentration of 1% ( W / V ) in the HA ethanol solution at 180 rpm for 1.5 h, then placed the PLA scaffold in absolute ethanol and ultrasonicated for 3 min to remove excess HA on the surface of the scaffold, and finally air-dried at room temperature.
[0056] (4) Weigh 10 g of gelatin and dissolve it in 100 mL of PBS solution, and stir for 1 h in a warm bath at 45 °C until completely dissolved. Slowly add 8 mL of MA into the gelatin solution dropwise, and after stirring for 2 h, add 92 mL of PBS solution at the same temperature and continue stirring for 15 min to obtain GelMA solution. The GelMA solution was transferred to a dialysis bag with a molec...
Embodiment 2
[0063] (2) Using FDM technology, biomimetic PLA scaffolds with gradient pore sizes of 400 μm and 800 μm were prepared by 3D printers.
[0064] (3) At room temperature, soak the PLA scaffold in an ammonia solution with a pH value of 12 and stir it at a speed of 175 rpm for 4 hours, and then soak the alkali-treated PLA scaffold in a concentration of 1% ( W / V ) in the HA ethanol solution at 180 rpm for 2 h, then placed the PLA scaffold in absolute ethanol and sonicated for 3 min to remove excess HA on the surface of the scaffold, and finally air-dried at room temperature.
[0065] (4) Weigh 10 g of gelatin and dissolve it in 100 mL of PBS solution, and stir in a warm bath at 50 °C for 1 h until completely dissolved. 8 mL of MA was slowly added dropwise to the gelatin solution, and after stirring for 1.5 h, 92 mL of PBS solution at the same temperature was added and stirred for 15 min to obtain GelMA solution. The GelMA solution was transferred to a dialysis bag with a molecular ...
Embodiment 3
[0072] (2) Using FDM technology, biomimetic PLA scaffolds with gradient pore sizes of 450 μm and 850 μm were prepared by 3D printers.
[0073] (3) At room temperature, soak the PLA scaffold in an ammonia solution with a pH value of 12 and stir at a speed of 180 rpm for 4 hours, and then soak the PLA scaffold after alkali treatment in a concentration of 1% ( W / V ) in the HA ethanol solution at 180 rpm for 1.5 h, then placed the PLA scaffold in absolute ethanol and ultrasonicated for 3 min to remove excess HA on the surface of the scaffold, and finally air-dried at room temperature.
[0074] (4) Weigh 10 g of gelatin and dissolve it in 100 mL of PBS solution, and stir for 1 h in a warm bath at 45 °C until completely dissolved. Slowly add 8 mL of MA into the gelatin solution dropwise, and after stirring for 2 h, add 92 mL of PBS solution at the same temperature and continue stirring for 15 min to obtain GelMA solution. The GelMA solution was transferred to a dialysis bag with a ...
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