Portable integrated POCT nucleic acid rapid detector

Abstract

The invention discloses a portable integrated POCT nucleic acid rapid detector. The portable integrated POCT nucleic acid rapid detector comprises a base; a center column is provided with a first containing chamber, one end of the center column is fixed to the base, and the other end of the center column can be selectively opened or closed; a turntable is rotatably arranged on the central column in a sleeving manner; 4 reagent release devices are movably arranged on the central column at intervals and used for releasing reagents; 8 chip clamping grooves are formed in the turntable at intervals and selectively communicate with the 4 reagent release devices; each chip clamping groove comprises: a groove is formed in a first shell and used for placing a micro-fluidic chip; a heating aluminum block is arranged at a bottom part of the groove and used for heating the micro-fluidic chip; a waste liquid cavity micropump interface communicates with a waste liquid cavity of the micro-fluidic chip and is used for maintaining negative pressure for the waste liquid cavity of the micro-fluidic chip; and a detection cavity micropump interface communicates with a detection cavity of the micro-fluidic chip and is used for maintaining negative pressure for the detection cavity of the micro-fluidic chip. The portable integrated POCT nucleic acid rapid detector has characteristics of short detection time and simple operation.

Description

technical field [0001] The present invention relates to the technical field of medical equipment, and more specifically, the present invention relates to a portable integrated POCT nucleic acid rapid detection instrument. Background technique [0002] Viral nucleic acid detection plays a vital role in the investigation of the source of infection. However, due to the large size and complicated operation of nucleic acid extraction and detection instruments, the samples taken at the epidemic site need to be transferred to large-scale hospitals after a long period of cold chain transportation. In the laboratory, during the long-term transportation, the samples have the risk of high temperature exposure, cross-infection and sample contamination, and the transportation also increases the waiting time of patients. [0003] At present, nucleic acid detection is divided into two parts: nucleic acid extraction and nucleic acid detection. It relies on large laboratories and requires la...

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Problems solved by technology

[0002] Viral nucleic acid detection plays a vital role in the investigation of the source of infection. However, due to the large size and complicated operation of nucleic acid extraction and detection instruments, the samples taken at the epidemic site need to be transferred to large-scale hospitals after a long period of cold chain transportation. In the laboratory, during the long-term transportation, the samples have the risk of high temperature exposure, cross-infection and sample contamination, and the transportation also increases the waiting time of patients

[0003] At present, nucleic acid detection is divided into two parts: nucleic acid extraction and nucleic acid detection. It relies on large laboratories and requires large precision instruments to operate independently. The waiting time does not allow rapid detection of the epidemic site

[0004] In order to ensure the expansion efficiency of nucleic acid detection, meet the requirements of fast and simple results, simplify the process, and reduce the volume of the instrument, conventional PCR (repeated heating and cooling requires a large centrifuge), ASBA (higher requirements for samples), RCA (reaction long time), HAD (high operating requirements) and RPA (high requirements for operators) and other nucleic acid amplification methods cannot be realized

[0005] In order to achieve a complete set of nucleic acid detection procedures "between square inches" at the epidemic site, conventional nucleic acid extraction methods include: phenol-chloroform extraction method (requires toxic reagents), magnetic particle method (needs to build a magnetic field), salting out method (except Impurities are not thorough) and glass bead method (residual reagents) can not meet the needs of on-site rapid extraction, and the requirements for the instrument are relatively high

[0006] In addition, similar products in the market have expensive testing equipment, complex operation, and long testing time, and it is difficult for grassroots medical institutions to popularize them. At the same time, the detection sensitivity of existing products is low, the interpretation is complicated, and there are problems such as missed detection, wrong detection, and delayed treatment.

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