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Preparation method of novel rumen bypass lipase

A lipase and rumen-passing technology, which is applied in the field of preparation of new rumen-passing lipase, can solve the problem of less utilization of the small intestine, and achieve the goal of increasing dry matter intake and milk production, increasing bioavailability, and increasing production capacity Effect

Active Publication Date: 2021-09-07
安徽东方天合生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the application of lipase in ruminants. This is mainly due to the direct addition of lipase, most of which are destroyed by microorganisms in the rumen and inactivated, and few of them can reach the small intestine and be utilized.

Method used

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  • Preparation method of novel rumen bypass lipase
  • Preparation method of novel rumen bypass lipase
  • Preparation method of novel rumen bypass lipase

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preparation example Construction

[0032] A preparation method of a novel rumen-passing lipase, comprising the steps of:

[0033] (1) Preparation of coating:

[0034] a. Place the methyl cellulose in the electron beam irradiation box for electron beam irradiation treatment, control the irradiation dose to 3~5MeV, and the irradiation treatment time is 2~3min, take it out for later use;

[0035] b. Weigh 10-12 parts of methyl cellulose, 20-24 parts of shellac, 18-22 parts of sodium alginate, and 35-45 parts of pure water after the treatment of operation a in the corresponding parts by weight, and place them in the micro-jet high-pressure homogenizer in turn. Carry out high-pressure homogenization treatment in the quality machine, control the working pressure at 80~90MPa, take out the homogenized product for later use;

[0036] c. Put the homogeneous product obtained in operation b into the dispersing cylinder for dispersing treatment. While dispersing, perform ultrasonic treatment of a specific frequency. Contro...

Embodiment 1

[0049] A preparation method of a novel rumen-passing lipase, comprising the steps of:

[0050] (1) Preparation of coating:

[0051] a. Place the methyl cellulose in the electron beam irradiation box for electron beam irradiation treatment, control the irradiation dose to 3MeV, and the irradiation treatment time is 2min, take it out for later use;

[0052] b. Weigh 10 parts of methyl cellulose, 20 parts of shellac, 18 parts of sodium alginate, and 35 parts of pure water after the operation a of weighing the corresponding parts by weight, and place them in the micro-jet high-pressure homogenizer in turn for high-pressure homogenization treatment , control the working pressure to be 80MPa, take out the homogeneous product for later use;

[0053] c. Put the homogeneous product obtained in operation b into the dispersing cylinder for dispersing treatment. While dispersing, perform ultrasonic treatment of a specific frequency, control the rotating speed of the dispersing cylinder t...

Embodiment 2

[0065] A preparation method of a novel rumen-passing lipase, comprising the steps of:

[0066] (1) Preparation of coating:

[0067] a. Place the methyl cellulose in the electron beam irradiation box for electron beam irradiation treatment, control the irradiation dose to 4MeV, and the irradiation treatment time is 2.5min, take it out for later use;

[0068] b. Weigh 11 parts of methyl cellulose, 22 parts of shellac, 20 parts of sodium alginate, and 40 parts of pure water after the operation a of weighing corresponding parts by weight, and place them in the micro-jet high-pressure homogenizer in turn for high-pressure homogenization treatment , control the working pressure to be 85MPa, and take out the homogeneous product for later use;

[0069] c. Put the homogeneous product obtained in operation b into the dispersing cylinder for dispersing treatment. While dispersing, perform ultrasonic treatment of a specific frequency, control the rotating speed of the dispersing cylinder...

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Abstract

The invention discloses a preparation method of novel rumen bypass lipase, and belongs to the technical field of enzyme preparations. The preparation method comprises the following steps of (1) preparing a coating; (2) preparing a core material; (3) coating treatment; (4) corona treatment; and (5) subzero treatment. According to the preparation method of the novel rumen bypass lipase provided by the invention, the finally prepared rumen bypass lipase is added into the daily ration of dairy cow, so that the dry matter intake and milk yield of the dairy cow can be remarkably improved, the bioavailability of the daily ration is improved, and the productivity is improved to a great extent.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparations, and in particular relates to a preparation method of a novel rumen-passing lipase. Background technique [0002] Dairy cows in a negative energy balance must use a large amount of body fat for energy in order to maintain milk production. The decomposition of body fat leads to weight loss of dairy cows, which limits the genetic potential of dairy cows for milk production and affects the reproductive performance of dairy cows. At the same time, It can also cause metabolic dysfunction in dairy cows, leading to diseases such as ketosis and fatty liver in dairy cows. In order to increase energy intake, the energy level of the diet must be increased. The commonly used method is to increase the feeding amount of concentrated grain feed. As a result, the concentration-to-coarse ratio of the diet is too high, the feed intake of roughage is seriously reduced, the type of rumen fermentation is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K40/35A23K50/10A23K20/189A23K20/158
CPCA23K40/35A23K50/10A23K20/189A23K20/158
Inventor 李寰旭
Owner 安徽东方天合生物技术有限责任公司