Nucleic acid detection method based on mass spectrometry
A detection method and nucleic acid technology, which is applied in the field of nucleic acid detection based on mass spectrometry technology, can solve the problems of fluorescence cross-color, detection channel limitation of flow fluorescence technology, and extremely complicated adjustment of fluorescence compensation, so as to achieve high resolution and reduce electric field penetration effect, the effect of improving ion flux and sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] figure 1 The flow chart of the nucleic acid detection method based on mass spectrometry technology in the embodiment of the present invention is given, such as figure 1 As shown, the nucleic acid detection method based on mass spectrometry technology is:
[0035] Designing corresponding nucleic acid probes according to the nucleic acid to be tested;
[0036] labeling the nucleic acid probe with a metal ion;
[0037] The nucleic acid probe labeled with metal ions enters the cell and hybridizes with the nucleic acid fragment to be detected in the cell;
[0038] Use mass spectrometry to detect single cells, obtain the content of metal ions in the cells, and obtain the information of the nucleic acid to be tested.
[0039] In order to improve the detection accuracy, further, the way of labeling the nucleic acid probe is:
[0040] Under the action of the cross-linking agent, metal ions and metal chelating agents are assembled; thiol modification of nucleic acid probes; ...
Embodiment 2
[0071] Application example of the nucleic acid detection method based on mass spectrometry technology in the detection of novel coronavirus according to Example 1 of the present invention.
[0072] In this application example, the nucleic acid detection method based on mass spectrometry technology:
[0073] Reagent preparation stage: According to the nucleic acid sequence of the new coronavirus, design and synthesize the corresponding probe sequence;
[0074] Using metal ions to label the nucleic acid probe, the specific method is: under the action of a cross-linking agent, the metal ion and the metal chelating agent are assembled; using TCEP to modify the nucleic acid probe with a sulfhydryl group;
[0075] Under the action of an appropriate buffer, the metal ion bound to the metal chelator binds to the sulfhydryl-modified nucleic acid probe;
[0076] Sample preparation stage: collect patient samples, use Ficoll method to separate blood samples, select peripheral blood cells...
Embodiment 3
[0104] Application example of the nucleic acid detection method based on mass spectrometry technology in the detection of novel coronavirus according to Example 1 of the present invention.
[0105] In a time-of-flight mass analyzer, such as Figure 5 As shown, the first grid 21 and the second grid 22 are grounded, which ensures the equipotential of the first grid 21 and the second grid 22; between the pulling electrode 12 and the first incident grid 31 there is a multi-hole that allows ions to pass through Electrodes, and use the voltage dividing resistor to divide the voltage of multiple electrodes, so that the electric field strength in the first ion acceleration area is uniform; the power supply applies positive pulse voltage to the repelling electrode 11, and applies negative pulse voltage to the pulling electrode 12;
[0106]The reflective area includes a first reflective field and a second reflective field, the first reflective field includes a second incident grid 32 an...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More