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Preparation method of novel maize superoxide dismutase multi-enzyme

A technology of dismutase multi-enzyme and superoxide, which is applied in the field of preparation of novel corn superoxide dismutase multi-enzyme, can solve the problems of easy residue of chemical disinfectants, large loss of enzyme activity, low enzyme yield and the like, and is not easy to achieve. The effect of breeding microorganisms, uniform temperature and humidity conditions, and high content of complex enzymes

Pending Publication Date: 2021-09-10
LIAONING PROSPECTIVE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many methods for preparing superoxide dismutase compound enzymes from corn, but there are problems of using chemical disinfectants such as bleaching powder and sodium hypochlorite in the preparation process. Because chemical disinfectants are easy to remain and pollute the environment during the production process, it is difficult for the preparation process. The quality of the superoxide dismutase compound enzyme also has a certain influence
At the same time, in the process of drying products, the existing methods adopt freeze-drying or spraying, the cost of the former is high, and the loss of enzyme activity of the latter is relatively large, neither of which is suitable for the needs of large-scale production
The existing method also has technical problems such as low germination rate, low enzyme yield, and small production scale. Since the corn is germinated in the box body of the germination device, there is a problem of small ventilation, and the growth environment of the corn is narrow, and it is easy to make the corn surface Bacterial film is produced, and corruption occurs, which leads to a decrease in germination rate, affects the quality of the obtained superoxide dismutase, and is prone to residues, is not easy to clean, and increases the labor intensity of workers
[0003] Chinese patent, the patent number is CN201210160871.0, and the name of the invention is an industrial production method for preparing superoxide dismutase compound enzyme from corn and its germination chamber. This patent solves the above technical problems, but it still has the following technical problems : The germination rate and yield are low, the concentration efficiency is low, the type of compound enzyme produced is not clearly indicated, and the shelf life of the compound enzyme at high temperature is not indicated

Method used

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  • Preparation method of novel maize superoxide dismutase multi-enzyme
  • Preparation method of novel maize superoxide dismutase multi-enzyme
  • Preparation method of novel maize superoxide dismutase multi-enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Prepare 2000kg of corn, remove impurities in the corn, and set aside; put the corn into a fully automatic seed cleaning, soaking, and germination device, wash, soak for 6 hours, set the temperature at 28-35°C, and germinate for 24 hours. Pass compressed air into the device every 0.5 hours, spray water into the device every 1 hour, drain the water after each spray, germinate until the buds are 3mm long, clean the germinated corn 3-5 times, and set aside. Add purified water to the germinated corn in a ratio of 1:3, grind the mixture evenly into corn steep liquor, stir the corn steep liquor evenly and filter, collect the filtrate, add 0.5% apple polyphenols by weight of the filtrate to the filtrate. Put the filtrate into a low-temperature vacuum concentrator, and evaporate it at 22-25°C for 8 hours to obtain a corn superoxide dismutase multi-enzyme liquid, mix the corn superoxide dismutase multi-enzyme liquid with starch evenly, and put it into In a drying oven, dry for 6 ...

Embodiment 2

[0032] Prepare 2000kg of corn, remove impurities in the corn, and set aside; put the corn into a fully automatic seed cleaning, soaking, and germination device, wash, soak for 10 hours, set the temperature at 28-35°C, and germinate for 50 hours. Pass compressed air into the device every 0.5 hours, spray water into the device every 1 hour, drain the water after each spray, germinate until the buds are 4mm long, clean the germinated corn 3-5 times, and set aside. Add purified water to the germinated corn in a ratio of 1:3, grind the mixture evenly into corn steep liquor, stir the corn steep liquor evenly, filter, collect the filtrate, and add 1% vitamin C of the filtrate weight to the filtrate. Put the filtrate into a low-temperature vacuum concentrator, and evaporate it at 22-25°C for 12 hours to obtain a corn superoxide dismutase multi-enzyme liquid, mix the corn superoxide dismutase multi-enzyme liquid and polysaccharide evenly, and put it into In a drying box, dry for 8 hour...

Embodiment 3

[0034]Prepare 2000kg of corn, remove impurities in the corn, and set aside; put the corn into a fully automatic seed cleaning, soaking, and germination device, wash, soak for 15 hours, set the temperature at 28-35°C, and germinate for 64 hours. Pass compressed air into the device every 0.5 hours, spray water into the device every 1 hour, drain the water after each spray, germinate until the buds are 5mm long, clean the germinated corn 3-5 times, and set aside. Add purified water to the germinated corn according to the ratio of 1:3, grind the mixture evenly into corn steep liquor, stir the corn steep liquor evenly and filter, collect the filtrate, and add 1.5% apple polyphenols by weight of the filtrate to the filtrate. Put the filtrate into a low-temperature vacuum concentrator, and evaporate it at 22-25°C for 15 hours to obtain a corn superoxide dismutase multi-enzyme liquid, mix the corn superoxide dismutase multi-enzyme liquid with starch evenly, and put it into In a drying...

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Abstract

The invention discloses a preparation method of novel maize superoxide dismutase multi-enzyme, and relates to the technical field of superoxide dismutase multi-enzyme preparation. The method comprises the steps of material preparation, germination, pulping, concentration, drying and the like. Maize in Liaoning area is selected, and the compound enzyme content is high; a full-automatic seed cleaning, soaking and germinating device is adopted, so that the treatment capacity is large, 2t of seeds can be germinated each time, the labor intensity is reduced, the bud length is uniform, the compound enzyme content is higher than that of a common germinating mode, and large-scale industrial production can be realized; a low-temperature vacuum concentrator is adopted to remove moisture at 22-25 DEG C for concentration, and at the temperature, the compound enzyme activity yield is higher and the concentration multiple is higher. Experiments show that when the product is stored at 45 DEG C, the enzyme activity is not reduced within 90 days, and when the product is stored at room temperature, the enzyme activity is not reduced within 48 months.

Description

technical field [0001] The invention relates to the technical field of superoxide dismutase multi-enzyme production, in particular to a preparation method of a novel corn superoxide dismutase multi-enzyme. Background technique [0002] The main effect of superoxide dismutase multi-enzyme on the human body is to effectively remove superoxide anions in the body - free radicals. Excessive free radicals stimulate cell proliferation changes and genetic factor mutations, which can cause various diseases. Reduce cell damage or death in the body, thereby reducing disease occurrence and delaying aging. There are mainly two types of existing methods for producing SOD multienzyme, one is extraction from animal viscera, and the other is extraction from plants such as corn. There are many methods for preparing superoxide dismutase compound enzymes from corn, but there are problems of using chemical disinfectants such as bleaching powder and sodium hypochlorite in the preparation process...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N9/08C12N11/10A01G31/02A01G31/00
CPCC12N9/0089C12N9/0065C12N11/10A01G31/02A01G31/00C12Y115/01001C12Y111/01009C12Y111/01006Y02P60/21
Inventor 王建英
Owner LIAONING PROSPECTIVE BIOTECH