Application of Querceric Acid in the Preparation of Drugs for Treating Pancreatic Cancer
A technology of quercus acid and pancreatic cancer, applied in the application field of medicine, can solve the problems of poor efficacy of pancreatic cancer and the like, and achieve the effect of inducing cell apoptosis
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Embodiment 1
[0021] Example 1 The effect of quercetin on the activity of pancreatic cancer cells PANC-1
[0022] Human pancreatic cancer cells PANC-1 were divided into 0.2×10 5 The density of cells / ml was inoculated into 35mm petri dishes, 2ml per dish, and placed in 5% CO. 2 24h in a 37°C incubator. The control group and the experimental group were set up. Quercus acid was dissolved in DMSO, and the DMSO did not exceed 0.2%. The concentrations of quercetin in the experimental group were 0.5 μM, 1 μM, 2 μM, 5 μM, and 10 μM, respectively. After administration, the cells were kept in the incubator for 72 hours, and the cell viability was detected by trypan blue exclusion method. Cell viability (%)=(number of viable cells / total number of cells)×100%.
[0023] The experimental results are as figure 1 As shown, the survival rate of PANC-1 cells treated with different quercus acid decreased in a dose-dependent manner with increasing drug concentration. Compared with the control group, 0.5 ...
Embodiment 2
[0024] Example 2 Apoptosis analysis of quercetin on pancreatic cancer cell PANC-1
[0025] Human pancreatic cancer cells PANC-1 were divided into 0.2×10 5 The density of cells / ml was inoculated into 35mm petri dishes, 2ml per dish, and placed in 5% CO. 2 24h in a 37°C incubator. The control group and the experimental group were set, and the concentration of quercetin in the experimental group was 0.5 μM, 1 μM, 2 μM, 5 μM, and 10 μM, respectively. After administration, the cells were kept in the incubator for 72 hours, and the apoptosis was detected by propidium iodide staining and Caspase-3 activity.
[0026] The experimental results are as figure 2 As shown, the apoptosis and Caspase-3 activity of PANC-1 cells treated with different quercus acid increased in a dose-dependent manner with the increase of drug concentration. Caspase family plays a very important role in the process of mediating apoptosis, among which caspase-3 is the key executive molecule, which functions ...
Embodiment 3
[0028] Example 3 Quercus acid inhibits migration of PANC-1 cells
[0029] The migration effect of quercus acid on PANC-1 cells was investigated by cell scratch assay. In this experiment, the cell density was 1 x 10 6 Cells / well were seeded on a 6-well plate. When the cell density grew to more than 95%, the medium was removed, and the surface of the cells was scratched with a 200 μl sterile pipette tip and washed twice with PBS to remove floating cells. The control group (no drug) and the experimental group (5 μM) were set, the serum content in the culture medium was 1%, and the cells were cultured in an incubator for 24 hours.
[0030] The result is as image 3 As shown, the migration ability of PANC-1 cells was significantly inhibited after 24h of 5 μM Quercus acid treatment. image 3 A shows the micrographs of the scratch area at 0 h and 24 h for the control and 5 μM quercus acid-treated cells in the migration experiment. The migration rate can be calculated by using Ima...
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