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Grape leaf extract and preparation method thereof

A grape leaf extract and grape leaf technology are applied in the fields of food ingredients containing natural extracts, food science, pharmaceutical formulations, etc., and can solve the problems of simultaneous extraction and enrichment of active ingredient compounds, few research on grape leaves, waste, etc.

Inactive Publication Date: 2021-09-14
劲牌持正堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many domestic literatures on grape seeds and grape skins, but few studies on grape leaves, and there is no simultaneous extraction and enrichment of multiple active ingredient compounds in grape leaves. The preparation can be widely used in medicine. Natural plant raw materials in the fields of food, cosmetics, etc., which also lead to the underutilization of grape leaf resources and cause waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of preparation method of grape leaf extract, concrete steps are as follows:

[0028] (1) Harvest, remove impurities, and dry: select grape leaves of the sapphire variety, harvest them on the first day after frost, select non-grape leaf impurities, and dry them naturally in the shade.

[0029] (2) Soaking: Weigh 500g of grape leaves, add 6000mL of pure water, and soak for 1 hour.

[0030] (3) Extraction: the above medicinal liquid was extracted, the extraction temperature was 60° C., the extraction time was 8 hours, and the extraction frequency was 2 times.

[0031] (4) Filtration: filter the above medicinal liquid with a filter cloth, and collect the filtrate.

[0032] (5) Concentration: Concentrate the above-mentioned filtrate, the concentration temperature is 50° C., the single feed liquid concentration time is 4 hours, and the solid content of the concentrated liquid is 50%.

[0033] (6) Drying: The above-mentioned concentrated solution was vacuum-dried at l...

Embodiment 2

[0036] A kind of preparation method of grape leaf extract, concrete steps are as follows:

[0037] (1) Harvest, remove impurities, and dry: select grape leaves of the Yanqisan variety, harvest them on the second day after the frost, select non-grape leaf impurities, and dry them naturally in the shade.

[0038] (2) Soaking: Weigh 500 g of grape leaves, add 10000 mL of ethanol with a volume fraction of 40%, and soak for 1 hour.

[0039] (3) Extraction: extract the above medicinal liquid, the extraction temperature is 70° C., the extraction time is 2 hours, and the extraction frequency is 2 times.

[0040] (4) Filtration: filter the above medicinal liquid with a filter cloth, and collect the filtrate.

[0041] (5) Concentration: Concentrate the above-mentioned filtrate, the concentration temperature is 50° C., the single feed liquid concentration time is 4 hours, and the solid content of the concentrated liquid is 40%.

[0042] (6) Drying: The above-mentioned concentrated solu...

Embodiment 3

[0045] A kind of preparation method of grape leaf extract, concrete steps are as follows:

[0046](1) Harvest, remove impurities, and dry: select the grape leaves of the Yanqisan variety, harvest them on the second day after the frost, select out non-grape leaf impurities, and dry them naturally in the shade.

[0047] (2) Soaking: Weigh 500g of grape leaves, add 10000mL of pure water, and soak for 1 hour.

[0048] (3) Extraction: extract the above medicinal liquid, the extraction temperature is 70° C., the extraction time is 1 hour, and the extraction frequency is 2 times.

[0049] (4) Filtration: filter the above medicinal liquid with a filter cloth, and collect the filtrate.

[0050] (5) Concentration: Concentrate the above-mentioned filtrate, the concentration temperature is 60° C., the single feed liquid concentration time is 4 hours, and the solid content of the concentrated liquid is 45%.

[0051] (6) Drying: The concentrated solution was vacuum-dried at a low temperat...

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PUM

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Abstract

The invention discloses a grape leaf extract and a preparation method thereof. The grape leaf extract is a brown-red powder extract prepared by extracting and refining red grape leaves serving as raw materials, has the unique flavor of the grape leaves, and can be completely dissolved in water, ethanol and methanol with different volume concentrations; and the extract comprises more than or equal to 14% of total polyphenol, more than or equal to 0.3% of anthocyanin content, and more than or equal to 0.005% of resveratrol content.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a grape leaf extract and a preparation method thereof. Background technique [0002] Grapes are one of the most widely cultivated fruits in the world. The latest report of the International Organization of Vine and Wine (OIV) shows that in 2018, the global viticulture area was about 7.44 million hectares, and the viticulture area in China was about 875,000 hectares. The grape cultivation process is accompanied by the production of a large number of by-products. The amount of pruning waste in one growth cycle is about 1-7.5 tons / ha, mainly including grape leaves, tendrils, stems, etc. Such by-products are mostly discarded, burned or composted. This method not only wastes resources, but also pollutes the environment. Grape leaves are mostly used as cooking materials in Turkey, Greece and the Middle East. In the diet of the United States and some Arab countries, grape lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/87A61K8/9789A61Q19/00A23L33/105A61K127/00
CPCA61K36/87A61K8/9789A61Q19/00A23L33/105A61K2236/13A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2800/10A61K2800/805A23V2002/00A23V2200/30A23V2250/21
Inventor 袁咏红李名洁梅菊王玉陈志元孙代华李清安
Owner 劲牌持正堂药业有限公司