Method for preserving blood oranges by using bacillus amyloliquefaciens M73 fermentation product
A technology of amylolytic spores and fermentation products, which is applied to the preservation of fruits and vegetables, and the preservation and application of food ingredients as antimicrobials. It can solve the problems of production and operator losses, so as to promote accumulation, extend the preservation period, and increase economic value. Effect
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[0020] 1. Materials
[0021] Blood orange (Citrus sinensis L. Osbeck) was collected from Zizhong County, Sichuan Province, and the variety was "No. 2".
[0022] The preservation liquid is a fermentation product of Bacillus amyloliquefaciens M73, and its preparation method comprises the following steps:
[0023] Step 1: Inoculate the cultured seed liquid into the fermentation medium at a ratio of 1% by volume, and incubate at 30°C for 48 h on a shaker at 200 r / min.
[0024] Step 2: centrifuge the fermented liquid from step 1 at 5000 r / min for 5 min.
[0025] Wherein: in step 1, the seed liquid is obtained by the following method: first transfer the Bacillus amyloliquefaciens strain M73 to a PDA plate by streaking, and place it in an incubator for 24-48 hours at a constant temperature of 28-37°C; The obtained lawn of Bacillus amyloliquefaciens strain M73 is inoculated into PDA liquid medium, shaken at 28-37°C for 24-48 hours to obtain seed liquid;
[0026] In step one, the fe...
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