Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of cyclocarya paliurus leaf flavone extract in preparation of antibacterial agent and/or antibacterial agent

A technology of willow leaf flavonoids and antibacterial drugs, applied in the field of preparation of antibacterial drugs and/or antibacterial agents against drug-resistant bacteria by extracting flavonoids from leaves of Cyclocarya paliurus

Pending Publication Date: 2021-10-01
SICHUAN WANGE AGRI TECH CO LTD +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the use of Cyclocarya paliurana extract as a drug or preparation against drug-resistant bacteria, and there is no report on the use of Cyclocarya paliurus extract as a drug or preparation against methicillin-resistant Staphylococcus aureus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of cyclocarya paliurus leaf flavone extract in preparation of antibacterial agent and/or antibacterial agent
  • Application of cyclocarya paliurus leaf flavone extract in preparation of antibacterial agent and/or antibacterial agent
  • Application of cyclocarya paliurus leaf flavone extract in preparation of antibacterial agent and/or antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the preparation of the flavonoid extract of Cyclocarya paliurus leaf of the present invention

[0027] Weigh 500 g of Cyclocarya paliurus leaves dried in the shade at room temperature, add 10 times (g / g) amount of water after crushing, extract twice at 90°C for 90 min each time, after concentration and centrifugation, take the supernatant and concentrate again. Then add ethanol until the final concentration of ethanol in the system is 65% (v / v), mix evenly, centrifuge at 4800r / min, take the supernatant, remove ethanol until the liquid has no alcohol smell. Then the liquid was eluted with a polyamide resin column (100 g of polyamide resin as the filler), first washed with 5BV of water, then eluted with 5BV of 60% ethanol, and the eluate was collected. The collected eluate was recovered by ethanol until it was liquid extract, and dried in vacuum at 50° C. for 24 hours to obtain a powdery solid, ie flavonoid extract from Cyclocarya paliurus leaves, which was ...

experiment example 1

[0030] Experimental example 1, test of the bactericidal effect of flavonoids from the leaves of Cyclocarya paliurus to methicillin-resistant Staphylococcus aureus

[0031] 1. Strains and drugs

[0032] Strains: (1) Common Staphylococcus aureus (ATCC25923) was purchased from the American Type Culture Collection (ATCC); (2) Methicillin-Resistant Staphylococcus Aureus (Methicillin-Resistant Staphylococcus Aureus, MRSA) were isolated from West China Hospital of Sichuan University, provided by the Clinical Microbiology Laboratory: MRSA-1 (clinical number: 1911101191), MRSA-2 (clinical number: 1911081137), MRSA-3 (clinical number: 1911051296), MRSA-4 ( Clinical number: 1911051125), MRSA-5 (clinical number: 1911081165).

[0033] Drugs: the flavonoid extract powder of Cyclocarya paliurus leaves prepared in Example 1, penicillin sodium and oxacillin sodium (purchase commercially available products).

[0034] 2. Culture of bacteria

[0035] Medium: common Staphylococcus aureus (ATCC2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of a cyclocarya paliurus leaf flavone extract in preparation of an antibacterial drug and / or an antibacterial agent, wherein the bacteria are drug-resistant bacteria. Experiments prove that the cyclocarya paliurus leaf flavone extract has an obvious killing effect on the methicillin gold drug-resistant staphylococcus aureus strain, and the problem that the methicillin gold drug-resistant staphylococcus aureus strain is resistant to most antibacterial drugs is solved. The cyclocarya paliurus leaf flavone extract can be prepared into an antibacterial drug or an antibacterial agent aiming at drug-resistant bacteria, and has an excellent application prospect in solving the worldwide problem of drug-resistant bacteria infection.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to the use of flavonoid extracts from leaves of Cyclocarya paliurus in preparing antibacterial drugs and / or antibacterial agents for drug-resistant bacteria. Background technique [0002] As we all know, bacterial drug resistance has become a major public health problem worldwide, and the abuse of antibiotics is an important reason for bacterial drug resistance. Methicillin-resistant Staphylococcus aureus (MRSA) has multidrug resistance and is associated with high morbidity and mortality. It is the leading cause of necrotizing pneumonia, severe sepsis, and necrotizing fasciitis The important pathogenic bacteria of the disease is one of the important pathogenic bacteria of hospital infection and community infection, which brings great difficulties to clinical treatment. MRSA is resistant to most antibacterial drugs or preparations, and even vancomycin, which is the most effectiv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P31/04A61K127/00
CPCA61K36/52A61P31/04A61K2236/331A61K2236/39A61K2236/53A61K2236/51A61Q17/00A61K8/9789A61K2236/333A61K2236/37
Inventor 孙美灵刘杰廖文廖敏钧
Owner SICHUAN WANGE AGRI TECH CO LTD