Nucleic acid extractor heating components

Abstract

The invention discloses a heating assembly of a nucleic acid extraction instrument, which includes a base for supporting a nucleic acid extraction kit, a first heating body and a second heating body are arranged on the base, and the first heating body corresponds to the test tube of the lysis station of the kit Setting, the second heating body is set corresponding to the test tube of the elution station of the kit, the first heating body is formed with a first heating surface, and the first heating surface is close to the test tube of the cracking station, so as to treat the liquid in the test tube of the cracking station. Heating, the second heating body is formed with a second heating surface, the second heating surface is close to the test tube of the elution station, so as to heat the liquid in the test tube of the elution station, the height of the first heating surface is greater than that of the second heating surface the height of. The heating assembly of the nucleic acid extractor of the present invention better matches the heating requirements of the lysing step and the elution step, improves the efficiency and quality of nucleic acid extraction, and the heating assembly is less likely to be displaced or misaligned up and down, and the matching accuracy with the kit is high.

Description

technical field [0001] The invention relates to the field of genetic engineering instruments and equipment, in particular to a heating assembly of a nucleic acid extraction instrument. Background technique [0002] Nucleic acid extraction instrument is an instrument that uses supporting nucleic acid extraction reagents to automatically complete sample nucleic acid extraction. It is widely used in disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety testing, animal husbandry and molecular biology. various fields of research. Nucleic acid extraction includes the following basic steps: 1. Cleavage: add lysate to the sample, and through mechanical movement and heating, the reaction solution is mixed and fully reacted to lyse the cells and release nucleic acid; 2. Adsorption: lyse the sample Add magnetic beads to the solution, mix well, use the magnetic beads to have a strong affini...

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Problems solved by technology

[0004] However, due to the large difference in the amount of reagents used in the two steps of cleavage and elution, the heating requirements of the two steps are different, and the existing heating device has the same heating structure and heating method for the cleavage and elution steps , which has resulted in that for the cracking step, the heating effect of the heating device is obviously insufficient, and for the elution step, the heating effect of the heating device is obviously excessive, so many problems have arisen: 1. In the cracking step, the lysate The amount used is usually about 300ul, and the amount of liquid is large. The heating device needs to continue heating for a long time to make the temperature meet the cracking requirements. Not only the heating efficiency is low, but also the overall speed of nucleic acid extraction will be affected; 2. Due to the cracking step required The heating time is longer, and the heat in the cracking area will radiate more to the nearby experimental areas such as washing and elution, which will affect the experimental results of other steps; 3. In the elution step, the amount of eluent used is usually about 50ul. The amount of liquid is small. For the eluent, using the same heating device as the cracking step will lead to excessive heating, resulting in excessive volatilization of the eluent, which not only wastes reagents, but also affects the concentration of reagents; 4. Some heating devices The height is too high, which is higher than the liquid level of the eluent. Therefore, during the oscillation process, if the magnetic beads are oscillated above the liquid level of the eluent, they are easily evaporated and dried on the inner wall of the test tube due to heat, resulting in The nucleic acid cannot be dissolved in the eluent normally, affecting the quality of the sample

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