Mycobacterium tuberculosis acid-fast staining quality control sheet/standard sheet and preparation method thereof
A Mycobacterium tuberculosis, acid-fast staining technology, applied in the field of medical testing, can solve the problems of poor simulation, poor stability and uniformity of quality testing, and difficulty in forming normal flora, and achieve good stability and uniformity. Small impact and improved standardization effect
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Embodiment 1
[0052] Example 1, Mycobacterium tuberculosis acid-fast staining quality control sheet / standard sheet is prepared through the following steps.
[0053] S1. Select healthy adult pigs (150-200 days old), extract bronchus and alveolar secretions from their bodies, and use them as cell simulation components. The specific extraction method is as follows:
[0054] S1-1. Bring the objects to the side of the breeding pig, and put the breeding pig down to a lying position.
[0055] S1-2. Turn on the power, turn on the intake switch of the suction device, and adjust the negative pressure.
[0056] S1-3. Connect the hose of the aspirator to the suction tube, and try to suck the normal saline to check whether it is unobstructed.
[0057] S1-4. Turn the pig to the operator lying on its back or on its side, and lean back slightly to allow it to open its mouth.
[0058] S1-5. Fold the end of the catheter with one hand to avoid damage to the mucosa caused by negative pressure adsorption.
...
Embodiment 2～17
[0075] Examples 2-17, Mycobacterium tuberculosis acid-fast staining quality control sheets / standard sheets, are different from Example 1 in that the composition of human simulated flora has been adjusted, as shown in Table 2 for details.
[0076] Select bacterial classification in table 2, embodiment 2~17 and select
[0077]
[0078] Among them, different bacterial suspension configuration methods are as follows:
[0079] Bacillus subtilis suspension is obtained by configuring as follows:
[0080] Purchase commercially available Bacillus subtilis freeze-dried powder, and before culturing the strain, all operations should be performed under biosafety protection and aseptic conditions.
[0081] Before opening the strain, disinfect the surface of the vial with 75% alcohol cotton. Under sterile conditions, open the plastic cover according to the direction of the arrow on the aluminum cover, tear off the aluminum cover, open the rubber stopper of the vial, add about 0.5mL of...
Embodiment 18～26
[0098] Examples 18-26, Mycobacterium tuberculosis acid-fast staining quality control sheet / standard sheet, differ from Example 11 in that the amount of the cell-simulating components and human flora-simulating components is shown in Table 3.
[0099] Table 3. List of dosage of each component (μL)
[0100] Example number Artificial sputum dosage Cell Mimetic Component Dosage Dosage of human simulated flora components Example 18 100 30 30 Example 19 100 50 100 Example 20 100 20 80 Example 21 100 80 20 Example 22 100 100 100 Example 23 50 50 50 Example 24 80 60 30 Example 25 50 30 50 Example 26 100 100 50
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